益肾调督针灸法对AD模型大鼠海马线粒体COXⅣ的影响  

Effect of Yi-Shen-Tiao-Du Acupuncture-Moxibustion on Hippocampus Mitochondria of COXⅣ in Alzheimer's Disease Rat

作　　者：崔翔[1] 马冉[1] 唐萁[1] 李丹[1] 杜艳军[1] 孔立江 孙国杰[1] 齐凤军[1] 

机构地区：[1]湖北中医药大学,湖北武汉430065

出　　处：《成都中医药大学学报》2014年第2期29-32,共4页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(编号:81273864;81173323)

摘　　要：目的:观察益肾调督针灸法对AD大鼠海马线粒体COXⅣ的影响,探讨针灸防治AD的具体作用机制。方法:将Wistar大鼠按随机数字表分为4组,即正常组、假手术组、模型组和针灸治疗组,采用双侧海马注射Aβ1-42的模型复制方法,针灸治疗组采用先针刺再艾灸"百会"和"肾俞"穴,通过免疫组化法观察大鼠海马线粒体中COXⅣ的表达水平。结果:大鼠行为学观察可见针灸治疗组大鼠进食量及饮水量正常,对外界刺激反应尚可,较模型组有所改善。同时通过免疫组化法对大鼠海马线粒体中COXⅣ平均光密度及平均灰度的统计学分析发现,模型组COXⅣ的表达明显低于正常组和假手术组(P<0.01),而针灸治疗组COXⅣ的表达显著性提高,与模型组比较具有统计学意义(P<0.01)。结论:益肾调督针灸法可以有效地提高海马线粒体COXⅣ表达,从而改善线粒体呼吸链功能,增加ATP产生,减少线粒体损伤,进而在AD防治中发挥作用。Objective： To observe the effects of Yi-shen-tiao-du acupuncture-moxibustion on the AD （Alzheimer＇ s disease） rats with hippocampus mitochondria COXⅣ, and to investigate the specific mechanism of preventive-treatment on AD by acupuncture- moxibusfion. Methods ： Wistar rats were randomly divided into 4 groups ： the normal group, sham-operated group, model group, and acupuncture-moxibustion group. First, bilateral hippocampus were injected Aβ1-42 to make rat model of AD. Then, acupuncture-moxi- bustion group selected Baihui points and Shenshu points, and treated with acupuncture and moxibustion. Last, detect the expression of hippocampus mitochondria COXⅣ in rats by the method of immunohistochemistry. Results： With rat behavior-observed, the acupunc- ture-moxibusition group which reacted by external stimuli could still, and eating-drinking normally, compared with the model group improved. Meanwhile, through immunohistochemistry analysis of average pptical and average gray about the expression of COX Ⅳ in hippocampalnerons mitochondfia, it could be found that compared with normal group and sham-operated group, COXⅣ expression of model group＇ s hippocampus mitoehondria is lower obviously than that of them （ P 〈 0. 01 ） . However, compared with the modelgroup, COXⅣ expression of acupuncture-moxibustion group＇ s hippocampus mitochondria increased significantly （P 〈 0. 01 ）. Con- clusion： Yi-shen-tiao-du anupuncture-moxibustion can increase the activity of hippocampapal mitochondrial respiratory chain enzyme complexe and ATP concentriation improve mitochondrial function. Thereby Yi-shen-tiao-du acupuncture-moxibustion plays a role in treatment and prevention for Alzheimer＇ s disease.

关 键 词：益肾调督针灸法 AD 线粒体 COXⅣ 

分 类 号：R245.3[医药卫生—针灸推拿学] R245.8[医药卫生—中医临床基础]