甲状腺乳头状癌组织SDF-1和TFF3表达临床意义分析  

Expression and clinical significance of SDF-1 and TFF3 in papillary thyroid carcinoma tissue

作　　者：杨建明[1] 黄静[1] 薛刚[1,2] 张静[1] 张文静[1] 吴靖芳[1] 

机构地区：[1]河北北方学院基础医学院,河北张家口075000 [2]河北北方学院附属第一医院耳鼻喉科,河北张家口075000

出　　处：《中华肿瘤防治杂志》2014年第12期914-919,共6页Chinese Journal of Cancer Prevention and Treatment

基　　金：河北省教育厅重点项目(ZD2010101);河北省科技厅项目(12276104D-91);河北省卫生厅项目(zd20133052)

摘　　要：目的:分析三叶因子3(trefoil factor 3,TFF3)和基质细胞诱导因子-1(stromal cell derived factor 1,SDF-1)在甲状腺乳头状癌(papillary thyroid carcinoma,PTC)和癌旁组织中的表达及意义,为PTC的早期诊断及预后评价提供有价值的资料。方法:采用免疫组织化学和原位杂交技术检测组织芯片(PTC和癌旁各31例)内TFF3、SDF-1蛋白和mRNA的表达,以SPSS 18.0分析其阳性率和平均光密度值与临床病理特征的关系。结果:1)PTC中TFF3蛋白阳性率为83.87%,明显高于癌旁组织25.81%,χ2=21.10,P<0.001;TFF3阳性率与TNM分期(χ2=12.30,P<0.001)和淋巴结转移(χ2=5.589,P=0.043)有关。PTC内TFF3的平均光密度(average optical density,AOD)值为0.49±0.01,高于癌旁0.22±0.06,t=3.448,P<0.001;有淋巴结转移者为0.57±0.06,高于无淋巴结转移者0.44±0.05,t=2.234,P=0.039;临床Ⅲ/Ⅳ期为0.59±0.07,高于Ⅰ/Ⅱ期0.47±0.05,t=2.167,P=0.041。2)PTC中SDF-1蛋白阳性率为87.10%,高于癌旁组织16.13%,χ2=31.26,P<0.001;有淋巴结转移者为100.00%,高于无淋巴结转移者75.00%,χ2=4.306,P=0.041;临床Ⅲ/Ⅳ期阳性率为100.00%,明显高于临床Ⅰ/Ⅱ期63.64%,χ2=8.35,P=0.04;>45岁为100.00%,明显高于≤45岁63.64%,χ2=8.35,P=0.04。癌内SDF-1的AOD值为0.59±0.07,高于癌旁0.28±0.08,t=3.987,P=0.000 7;有淋巴结转移者为0.65±0.06,高于无淋巴结转移者0.52±0.05,t=2.458,P=0.009;临床Ⅲ/Ⅳ期为0.66±0.06,高于Ⅰ/Ⅱ期0.50±0.05,t=2.762,P=0.006。3)原位杂交显示,TFF3mRNA和SDF-1mRNA与蛋白表达一致,阳性率虽低于相应蛋白(χ2=19.37,P<0.001),但明显高于癌旁组织,χ2=26.35,P<0.001。4)PTC中TFF3与SDF-1蛋白表达水平呈正相关,r=0.971,P=0.004。5)31例PTC可区分为典型PTC 21例(67.74%)、滤泡型7例(22.58%)和高细胞型3例(9.68%)3个亚型。SDF-1和TFF3蛋白阳性率依次为典型PTC(86.96%与86.96%)、滤泡型PTC(85.71%与71.43%)和高细胞型PTC均为100.00%,不同亚型间SDF-1和TFF3阳性率差异无统计学意义;临床Ⅰ/Ⅱ期滤泡�OBJECTIVE： To investigate the expression and significance of TFF3 and SDF 1 in papillary thyroid carcinoma（PTC） and para-carcinoma tissues,and provide valuable data for early diagnosis and prognosis of PTC. METHODS： Immunohistochemieal（IHC） SP and in situ hybridization（ISH） were used to detect the expression of TFF3,SDF-1 proteinand mRNA in tissue chip including 31 cases of PTC and para-carcinoma tissues. The positive rate,average optical density （AOD） and the relationship with clinicopathologic features were analyzed by SPSS 18.0. RESULTS： The positive rate of TFF3 protein in PTC was higher than that in para-carcinoma tissues （83.87% vs 25.81% ,x2 =21.10,P〈0. 001）. The positive rate of TFF3 protein in patients with PTC was correlated with the TNM stage and metastasis in lymph nodes （x2 =12.30,P〈0. 001;x2 =5. 589,P=0. 043）. The AOD values of TFF3 was higher in PTC than that in para-carcinoma （0.49±0.01 vs 0. 22±0.06;t=3. 448,P〈0. 001） ,and it was higher in cases of lymph node metastasis than those without metastasis （0.57±0.06 vs 0. 44±0.05;t=2. 234,P=0. 039）. AOD value of TFF3 was higher in stage Ⅲ-Ⅳ than those in stageⅠ/Ⅱ （0.59±0.07 vs 0. 47 ± 0.05 ; t = 2. 167, P = 0. 041 ）. The positive rate of SDF-1 protein in PTC was higher than that in para-carcinoma tissues （87.10 % vs 16.13 % ;X2 = 31.26, P〈0. 001）, in cases of lymph node metastasis was higher than that without metastasis（100.00% vs 75.00% x2 =4. 306,P=0. 041） ,it was higher in stageⅢ-Ⅳ than stageⅠ/Ⅱ（100.00% vs 63.64%;3（2 =8.35,P=0.04）,it was higher in age over 45 years old than that under 45 （100.00% vs 63.64% x2 =8. 35,P=0. 04）. AOD values of SDF-1 protein in PTC was higher than that in para-carcinoma （0.59±0.07 vs 0. 28±0.08±0. 087,P〈0. 001 7） ,in cases of lymph node metastasis was higher than that without metastasis（0.65±0.06 vs 0.52±0. 05;t=2.458,P=0. 009）,in stage Ⅲ-Ⅳ was higher than that in stageⅠ/Ⅱ （0.66± 0.06 vs 0. 50±0.05;t=2. 76

关 键 词：甲状腺肿瘤 病理亚型 三叶因子3 基质细胞诱导因子-1 免疫组织化学 原位杂交 组织芯片 

分 类 号：R736.1[医药卫生—肿瘤]