MicroRNA-1对肝癌细胞BAG4表达的影响  

Effects of miR-1 on gene expressing of BAG4 in hepatoma carcinoma cell line

作　　者：刘煜[1] 李华[2] 李东[2] 张涛[2] 

机构地区：[1]第三军医大学研究生管理大队,重庆400038 [2]成都军区总医院肿瘤诊治中心,610083

出　　处：《免疫学杂志》2014年第6期479-482,487,共5页Immunological Journal

基　　金：国家自然科学基金青年基金(81101634);成都军区"十二五"医学科学研究计划重点项目(B12018);四川省科技厅基金资助项目(2012SZ0058);四川省卫生厅科研课题资助项目(110472;110478)

摘　　要：目的观察microRNA-1(miR-1)对肝癌细胞系Hep3B死亡结构域沉默子(BAG4)的表达及细胞凋亡的影响。方法 Western blot检测肝癌组织中BAG4蛋白表达情况;将过表达miR-1的质粒转染肝癌细胞Hep3B,分别用RT-qPCR及Western blot检测BAG4 mRNA和蛋白的表达,流式细胞仪检测细胞凋亡;生物信息学软件分析miR-1和BAG4 3’UTR作用关系。结果 BAG4蛋白在肝癌组织中的表达明显高于癌旁组织;Hep3B转染miR-1过表达质粒后,相较于阴性对照(NC)组,BAG4mRNA和蛋白水平的表达均有所降低;早期凋亡率明显增高。生物信息学分析提示BAG4是miR-1潜在靶基因。结论 miR-1可以诱导肝癌细胞Hep3B凋亡,其作用可能和抑制BAG4表达有关。To investigate the effects of miR-1 on BAG4 expression and cell apoptosis in human hepatoma carcinoma cell line Hep3B, we detected the expressions of BAGS protein in HCC tissues and paired non-cancerous liver （PNL） tissues with Western blot. After miR-1 overexpressing plasmid was transfected into Hep3B, the expression levels of BAG4 mRNA and protein were measured by RT-qPCR and Western blot respectively, and the apoptosis ratio of Hep3B was detected by flow cytometry. The relationship of miR-1 and BAG4 were analyzed with bioinformatics method. Results showed that protein level of BAG4 in HCC tissue was much higher than that in PNL tissue. The miR-1 overexpressing group demonstrated much lower of BAG4 mRNA and protein levels, but higher level of cell apoptosis, as compared with negative control （NC） group. Bioinformatics analysis revealed BAG4 may be a potential target gene of miR-1. Therefore, we concluded miR-1 can induce apoptosis of Hep3B by downregulating BAGS expression.

关 键 词：肝癌 MICRORNA-1 死亡结构域沉默子 凋亡 

分 类 号：R735.7[医药卫生—肿瘤] R392.11[医药卫生—临床医学]