线粒体calpains与细胞凋亡关系的研究进展  

Role of mitochondrial calpains in apoptosis

作　　者：宋必卫[1] 樊俏玫[1] 何治宇[1] 

机构地区：[1]浙江工业大学药学院,浙江杭州310014

出　　处：《中国药理学通报》2014年第6期757-760,共4页Chinese Pharmacological Bulletin

摘　　要：calpains是一类由Ca2+激活的内源性半胱氨酸蛋白酶。尽管先前研究认为calpains是一种胞质酶,最近研究发现μ-calpain、m-calpain、calpain10以及内源性抑制剂calpastatin也同时存在于线粒体中,在caspase依赖型和非依赖型细胞凋亡通路中均发挥着重要的作用。calpain除了与caspase相互作用外,还可剪切凋亡相关蛋白如Bcl-2、Bax、Bid等,促进Cyt-C、AIF的释放,从而参与调控细胞凋亡的病理过程。calpains作为潜在的治疗靶点,研究线粒体calpains与细胞凋亡通路的关系具有重大意义。Calpains are the family of Ca2＋-activated cysteine pro-teases. Although calpains are considered to be cytoplasmic en-zymes, recent research has demonstrated that μ-calpain, m-calpain, calpain 10 and their endogenous inhibitor calpastatin are present in the mitochondria and play important roles both in caspase -dependent and-independent pathways in cell death phenomena. Calpains exert direct and indirect effects on the caspases and regulators of apoptosis pathway such as Bcl-2, Bax, Bid, promoting the release of Cyt-C, AIF, then result in cellular ap-optosis. To allow pharmacological targeting of these enzymes, thorough knowledge of their patterns of activation and further in-teractions with already known apoptotic pathways is necessary.

关 键 词：线粒体 钙离子 μ-calpain m—calpain calpain10 凋亡 

分 类 号：R05[医药卫生] R329.25