当归-红花不同配比对血虚小鼠补血作用的比较研究  

Comparing tonifying blood effects of Danggui-Honghua with different proportions on blood deficiency mice

作　　者：李淑娇[1] 唐于平[1] 李伟霞[1] 沈娟[1] 郭建明[1] 段金廒[1] 

机构地区：[1]南京中医药大学江苏省中药资源产业化过程协同创新中心/中药资源产业化与方剂创新药物国家地方联合工程研究中心,江苏南京210023

出　　处：《中国药理学通报》2014年第6期874-879,共6页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No 81274058);江苏高校优势学科建设工程项目(No ysxk-2010)

摘　　要：目的观察当归、红花两药味不同配比对血虚小鼠的补血作用差异,优选当归-红花配伍补血作用的最佳配比。方法采用乙酰苯肼与环磷酰胺联合造模法复制小鼠血虚模型。基于多指标综合指数法,首先以《中医方剂大辞典》数据库中出现频次最高的当归-红花1∶1配比为代表,优选当归、红花配伍补血作用的最佳浓度;然后采用优选的最佳浓度来观察当归、红花不同配比的补血作用变化规律。结果在3个不同浓度(分别为当归、红花临床等效量的1、3、5倍)下,当归-红花剂量为临床等效量时的总补血效应最好;在当归、红花的9个不同配比(1∶0、4∶1、2∶1、3∶2、1∶1、2∶3、1∶2、1∶4和0∶1)中,以当归-红花1∶1的总补血效应最好。结论与中医方剂中使用当归、红花1∶1频次最高的用药规律相一致,为现代临床更有效应用当归与红花提供了科学依据。Aim To observe the differences of tonify-ing blood effect for the combination of Danggui and Honghua （ GH） with different proportions on blood de-ficiency mice, and choose the proportion of GH which has the optimal tonifying blood effect. Methods The blood deficient mice model was induced by injection of phenylhydrazine and cyclophosphamide. On the basis of multi-attribute comprehensive index method, the op-timal dose was determined through three doses of GH 1： 1 , which was the highest frequency proportion of GH used in the “Dictionary of Chinese Medicine Prescrip-tions”. According to the optimal dose, the change regulation of tonifying blood effect of GH with different proportions was observed. Results Among three doses （1, 3 and 5 times of clinical dose）, the tonifying blood effect of GH was best when the dosing concentra-tion of GH was clinical dose. Among nine proportions （1 ： 0, 4 ： 1, 2 ： 1, 3 ： 2, 1 ： 1, 2 ： 3, 1 ： 2, 1 ： 4 and 0 ： 1）, GH 1 ： 1 had the best effect. Conclusion The results are consistent with regulations of medicine usage that GH 1 ： 1 has the highest frequency used in Herbal Formulae, which could provide scientific basis for more effective application of Danggui and Honghua in modern clinic medicine.

关 键 词：当归 红花 配比 血虚 养血补血 多指标综合指数法 

分 类 号：R-332[医药卫生] R255.7