三回波梯度回波序列与1H-MRS 在大鼠肝内脂肪含量测定中的应用  

The Application of Triple Echo Gradient Echo Sequence and 1H-MRS in Detection and Measurement Steatosis in Nonalcoholic Fatty Liver Disease Rat Model

作　　者：韩海伟[1] 韩成坤[1] 汪敬群[1] 庄碧梅[1] 陈识[1] 张惠杰[2] 李学军[2] 庄雄杰[1] 

机构地区：[1]厦门大学附属第一医院放射科,361003 [2]厦门大学附属第一医院糖尿病-内分泌科,361003

出　　处：《临床放射学杂志》2014年第6期929-932,共4页Journal of Clinical Radiology

摘　　要：目的探讨三回波梯度回波序列(简称三回波序列)及1H-MRS在非酒精性脂肪肝(NAFLD)大鼠肝内脂肪含量测定中的应用。方法 25只NAFLD S-D大鼠同时行三回波序列与1H-MRS检查测定肝内脂肪含量,同时将大鼠肝脏行组织病理学检查测定肝内脂肪含量,并将三回波序列测定结果与组织病理学结果加以对照。结果三回波序列及1H-MRS测定大鼠的肝内脂肪含量结果分别为16.8%±6.7、27.8%±9.9,二者呈明显强正相关(r=0.957,P<0.0001);组织病理学测得肝内大鼠脂肪含量为43.3%±18.8,与三回波序列测得肝内脂肪含量结果呈明显正相关性(r=0.984,P<0.0001),二者行Bland&Altman曲线分析显示一致性非常好。结论三回波序列能够在体、无创性的测定肝内脂肪含量,并且有望在肝内脂肪含量测定上取代组织病理学。Objective To study the value of triple echo gradient echo sequence and roll-MRS in detection and measur- ment steatosis in nonalcoholic fatty liver disease rat model. Methods Twenty five male NAFLD rat model and five S D rats served as control underwent MR triple echo gradient echo sequence and 1H-MRS examination. The content of the lipid was measured on MR and MRS, and the results were compared with histologic findings. Results The content of the lipid quantified by triple echo gradient echo sequence and ill-MRS was 16.8% ±6.7 and 27.8% ± 9.9, which had highly cor- relation （ r = 0. 957 ,P 〈 0. 0001 ）. The content of lipid quantified by histology was 43.3% ± 18.8, which had highly corre- lated with that quantified by triple echo gradient echo sequence （ r = 0. 984,P 〈 0. 0001 ）. The results of MR and histology had great agreement using Bland ＆ Ahman analysis Conclusion Triple echo gradient echo sequence can be used to accu- rately quantify fat in vivo in an animal model of hepatic steatosis and may serve as a quantitative hiomarker of hepatic steatosis.

关 键 词：磁共振成像 氢质子波谱 非酒精性脂肪肝 

分 类 号：R575.5[医药卫生—消化系统]