组织印片DNA倍体分析在胃癌诊断和预后评估中的应用  

DNA ploidy analysis of tissue imprint to gastric carcinoma diagnosis and the prognosis appraisal application

作　　者：李德昌[1] 岳颖[1] 郑吉春[1] 任力[1] 

机构地区：[1]空军总医院病理科,北京100142

出　　处：《空军医学杂志》2014年第1期48-50,共3页Medical Journal of Air Force

基　　金：首都卫生发展科研专项基金(2011-5041-01)

摘　　要：目的探讨组织印片DNA倍体分析对胃癌的诊断及预后评估的意义。方法用全自动图像分析系统分析138例胃癌组织及其周边组织的DNA倍体类型,以及与临床病理学特征的关系。结果 DNA倍体分析与病理诊断的符合率较高为92.0%(254/276),有异倍体的组织存在恶变可能性。胃癌中DNA异倍体发生率与性别、年龄及肿瘤大小、浸润深度无明显关系;而与分化程度及淋巴结转移有关。高分化腺癌与中分化及低分化腺癌的DNA异倍体发生率相比,有显著性差异(P<0.05)。淋巴结转移组DNA异倍体率与无淋巴结转移组有显著性差异(P<0.01)。结论 DNA倍体分析可作为胃癌筛选和早期诊断的重要指标及病理诊断辅助方法。DNA倍体异常与胃癌的生物学特性密切相关,可用于预后判断和疗效观察。ObjectiveWe attempted to study the significance of DNA ploidy analysis in gastric carcinoma diagnosis and itsprognosis appraisal.MethodsDNA ploidy of tumor tissue and the tumor peripheral tissue of 138 patients with gastric carcinoma was analyzed by automatic image cytomery, and analyzed its relationship to clinical pathology characteristics.ResultsThe coincidence of DNA ploidy analysis with pathological result was 92.0 %（254/276）, The normal tissues with aneuploid had high risk of cance ration. The incidence of DNA aneuploid wasn’t related to sex, age, tumor size or invasion depth; but to differentiation degree and the lymph node metastasis in the gastric carcinoma. The incidence of DNA aneuploid in high differentiated tumor is lower than that in middle and low differentiated tumor（P〈0.05）. The incidence of DNA aneuploid in tumor with lymph node metastasis was higher than that in tumor without lymph node metastasis （P〈0.01）.ConclusionsDNA ploidy analysis may be taken as an indication for gastric carcinoma screening and the early diagnosis important target, and as an auxiliary method of the pathological diagnosis. DNA aneuploid has close correlation with biologic characteristics of gastric carcinoma, which is available to patient＇s prognosis judgment and curative effect observation.

关 键 词：胃癌 DNA倍体分析 DNA异倍体 

分 类 号：R735.2[医药卫生—肿瘤]