青蒿素对实验性糖尿病大鼠肾脏蛋白激酶C移位活化的抑制作用  

Inhibitory effect of artemisinin on the spatiotemporal dynamics activation of protein kinase C in the kidney of experimental diabetic rats

作　　者：张丽坤[1] 张建勇[1] 周凤娇[1] 苏彦君[1] 安志霞 

机构地区：[1]河北省石家庄市第二医院,河北石家庄050051

出　　处：《现代中西医结合杂志》2014年第18期1964-1966,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

摘　　要：目的探讨青蒿素对实验性糖尿病大鼠肾脏蛋白激酶C(PKC)由细胞浆到细胞膜移位活化的抑制作用。方法将实验大鼠随机分为对照组(A组)、糖尿病非治疗组(B组)及糖尿病青蒿素治疗组(C组),B组、C组采用腹腔单剂量注射链脲佐菌素(STZ)65 mg/kg的方法建立糖尿病大鼠模型,造模后C组采用青蒿素300 mg/(kg·d)腹腔注射。于实验进行到第3周、第6周时分别宰杀大鼠6只,测算肌酐清除率(Ccr)、24 h尿白蛋白排泄率(UAER)及肾脏系数(肾脏质量/体质量);采用酶联免疫吸附法(ELISA)测定PKC的活性。结果 B组大鼠Ccr、UAER、肾脏细胞膜PKC活性及肾脏系数均较A组明显升高(P<0.05或0.01);C组大鼠上述指标均较B组明显下降(P<0.05或0.01)。结论青蒿素通过抑制糖尿病大鼠肾脏细胞PKC移位活化而对糖尿病大鼠早期肾脏病变起到保护作用。Objective It is to approach the inhibitory effect of artemisinin on the spatiotemporal dynamics activation of protein kinase C （PKC） in the kidney of experimental STZ - induced diabetic rats. Methods The experimental rats were divided into control group （group A） , diabetic without treatment group （group B） and diabetic with treatment of artemisinin group （group C）. The model of diabetic rats was established by intraperitoneal injection of a single close of 65 mg/kg induced by STZ. After modeling, the animals in group C were treated with artemisinin of 300 mg/（ kg·d） by intraperitoneal injection. UAER, Ccr and Kidney coefficient were observed after 3 - and 6 - week of treatment, while the endogenous PKC activity was measured by ELISA. Results Kidney coefficient, Ccr, UAER and PKC activity at the plasma membrane in group B were significantly higher than those in group A （P 〈 0.05 or 0.01 ）; meanwhile, these indexes in group C were markedly lower than those in group B （ P 〈 0.05 or 0.01 ）. Conclusion Artemisinin has some renoprotective effects in experimental STZ - induced diabetic rats through inhibiting the spatiotemporal dynamics activation of PKC in the kidney.

关 键 词：青蒿素 蛋白激酶C 移位活化 糖尿病肾病 

分 类 号：R-332[医药卫生]