通过序列分析筛选单纯疱疹病毒I型蛋白组中的PACS-1结合蛋白  

Screening for PACS-1 binding protein in herpes simplex virus-1 proteome by sequence analysis

作　　者：刘军[1] 李英辉[1] 薛采芳[1] 甄荣芬[1] 李旬[1] 王宪锋[1] 刘忠湘[1] 万磊[1] 

机构地区：[1]第四军医大学病原生物学教研室,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2001年第2期123-125,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助;No.39970038

摘　　要：目的筛选单纯疱疹病毒I型蛋白组中的PACS1结合蛋白。方法根据筛选标准,利用ScanProsite和PSORT软件对蛋白质进行分析,并结合相关文献中的实验数据对分析结果进行校正。结果该方法可有效地筛选出PACS1结合蛋白。利用该方法,在单纯疱疹病毒I型蛋白组中,共筛选出可能与PACS1结合的蛋白质33种。结论建立了一种有效的筛选PACS1结合蛋白的方法。筛选出的单纯疱疹病毒I型的PACS1结合蛋白,将为研究PACS1在疱疹病毒发生及潜伏性感染中的作用提供指导。Aim To screen for PACS 1 binding protein in HSV 1 proteome in order to direct experimental research. Methods HSV 1 proteins were analysed by ScanProsite and PSORT software according to 4 screening criteria, then the results were modified by experimental data from references. Results The screening methods are effective. Total 33 proteins from HSV 1 proteome are identified as possible PACS 1 binding proteins. Conclusion An effective method for screening PACS 1 binding protein are established. The identified possible PACS 1 binding proteins of HSV 1 will provide instructions for experimental research on the role of PACS 1 in biogenesis and latent infection of HSV.

关 键 词：单纯疱疹病毒Ⅰ型 PACS-1 蛋白组 序列分析 生物信息学 筛选 

分 类 号：R373.11[医药卫生—病原生物学]