浒苔纤维素分解菌株的筛选、鉴定与纤维素酶活特性研究  

Isolation and identification of Ulva prolifera cellulase-producing strains and characterization of their cellulase-producing capability

作　　者：费岚[1,2] 邵飞[1,2] 贾睿[1,2] 胡乐琴[1,2] 何培民[1,2] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]上海海洋大学海洋科学研究院,上海201306

出　　处：《上海海洋大学学报》2014年第3期411-416,共6页Journal of Shanghai Ocean University

基　　金：科技部支撑计划项目(2012BACD7B03)

摘　　要：从腐烂浒苔和浒苔暴发区域的海水中筛选出具有纤维素分解能力的菌株,采用刚果红染色法进行粗选,得到8株透明圈较大的菌株。将8株菌株分别接种到3种不同碳源的液体发酵培养基,发酵培养6 d后,分别测定滤纸(FPA)酶活力、羧甲基纤维素(CMC)酶活力与水解浒苔纤维素的效果。8株菌株中得到H3、H4、H6、Q1四种产酶较好的菌株并鉴定。3种液体发酵培养基中,以羧甲基纤维素钠为碳源的条件下,H3的CMC酶活力最高,为56.98 U/mL。H3与H4的浒苔水解效果较好,还原糖得率分别为10.4%和12.8%。Green tide outbreak results in massive Ulva prolifera accumulation in coast China. Because Ulva prolifera has high proportion of cellulose content,it can be used as the raw material for manufacturing bioethanol when cellulose degradation produces oligosaccharides. In this study,by using Congo red staining method,eight larger lysis zone strains which have the ability of cellulose-decomposing were obtained from rotted Ulva prolifera and green tide outbreak sea area. Cellulose enzyme activity characteristics and the ability to decompose Ulva prolifera of each strain were studied and high cellulose enzyme activity strains were identified. Enzyme activity of FPA and CMC in strains H3,H4,H6 and Q1 was higher than others,and carboxymethylcellulose as carbon source induced enzyme work best. Enzyme activity of CMC in the strain H3 was the highest（ 56. 98 U / mL）. And the best reducing sugar yield were 10. 4% and 12. 8% from strain H3 and strain H4. All the four strains were obtained by morphological identification and molecular biology identification. The experiments show that Ulva prolifera can be used as a raw material for preparation of bioethanol,and the screened strains can used for cellulose degradation.

关 键 词：浒苔 纤维素降解 菌株 酶活力 

分 类 号：S216.3[农业科学—农业机械化工程]