大豆磷脂酰乙醇胺的分离及纯化  

Separation and purification of soybean phosphatidylethanolamine

作　　者：王强[1] 史苏佳[2] 韩海霞[1] 曹栋[1] 

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]江南大学理学院,江苏无锡214122

出　　处：《中国油脂》2014年第6期72-77,共6页China Oils and Fats

基　　金："十二五"国家科技支撑计划(2012BAD33B05)

摘　　要：以提取卵磷脂(PC)后的大豆粉末磷脂为原料,以碱性乙醇为溶剂提取磷脂酰乙醇胺(PE),并进行冷冻纯化。在单因素实验的基础上,以PE得率为指标,响应面优化实验确定了PE的最佳提取条件为:提取温度45℃,料液比1∶20,乙醇碱浓度V(无水乙醇)∶V(25%氨水)=100∶2,提取时间50 min,提取次数2次。在最佳条件下,PE得率为79.53%,含量为48.42%;当冷冻温度为-15℃,冷冻时间为8 h时,PE含量提升到72.73%,得率为66.71%。With soybean phospholipids powder as raw material, in which phosphatidylcholine was extracted, phosphatidylethanolamine （ PE ） was extracted by alkaline ethanol and purified by freezing. With yield of PE as the indicator, based on the single factor experiment, the optimal extraction conditions of PE were obtained by response surface methodology as follows：extraction temperature 45℃, solid-liquid ratio 1：20,concentration of alkaline ethanol 2. 00 mL of 25% ammonia per 100 mL ethanol, extraction time 50 min and extraction times twice. Under these conditions, the yield and content of PE were 79. 53% and 48. 42% respectively. Meanwhile, under the conditions of freezing temperature -15℃and freezing time 8 h, the content of PE improved to 72. 73% and the yield was 66. 71%.

关 键 词：大豆粉末磷脂 磷脂酰乙醇胺 溶剂提取 冷冻纯化 

分 类 号：TS229[轻工技术与工程—粮食、油脂及植物蛋白工程] TQ645[轻工技术与工程—食品科学与工程]