原发性肝细胞癌中iNOS与p16、Bax的表达及其与细胞凋亡的关系  

Expressions of iNOS,p16,Bax,and its Association with Apoptosis in Primary Hepatocellular Carcinoma

作　　者：姜军梅[1] 朱菊人[1] 刘吉勇[1] 孙成刚[1] 陆伟君[1] 

机构地区：[1]山东省立医院,250021

出　　处：《实用癌症杂志》2001年第2期123-125,共3页The Practical Journal of Cancer

摘　　要：目的　探讨在原发性肝细胞癌 (HCC )中一氧化氮合酶 (iNOS)的表达对p16、Bax表达的影响及其与细胞凋亡的关系。方法　采用免疫组化的方法对HBsAg均阳性的 49例HCC患者的iNOS、p16、Bax表达进行定位和半定量分析。采用TUNEL法检测细胞凋亡。结果　iNOS和Bax以棕黄色颗粒着色于细胞质和 (或 )细胞膜中 ,细胞核阴性 ,间质细胞着色不明显。p16以棕黄色着色于细胞核中 ,细胞质中偶见。细胞凋亡为细胞核着色 ,细胞质阴性。p16、Bax的表达及细胞的凋亡率与iNOS的表达有关。结论　iNOS的表达可诱导抑癌基因 p16、Bax的表达和细胞凋亡的发生 ,从而影响HCC的分化。故在诱导HCC中iNOS的表达 。Objective To study the relations between the expression of iNOS,p16,Bax and apoptosis in primary hepatocellular carcinoma(HCC).Mathods 49 cases of paraffin embaded HCC tissue were studied for expression of iNOS,p16,Bax with immnohistochemistry method.Apoptosis was analyzed by Tunel method.Results iNOS and Bax protein appeared in the plasma or membrane of the cells.p16 appeared mostly in the nuclei.The apoptosis cells were identified by colored nuclear.The positive relation between iNOS expression and p16,Bax expression as well as apoptosis existed.Conclusion iNOS expression can induce the expression of p16,Bax and apoptosis in HCC,then it will affect the differentiation of HCC.Inducing iNOS expression may improve the clinical therapy effects of HCC.

关 键 词：肝肿瘤 一氧化氮酶 P16基因 BAX基因 细胞凋亡 原发性肝细胞癌 基因表达 

分 类 号：R735.7[医药卫生—肿瘤]