食管癌前病变及原位癌组织中Ki67、p53、iNOS的异常表达  

Abnormal expression of p53,Ki67 and iNOS in human esophageal carcimoma in situ and premalignant lesions

作　　者：靳玉兰[1] 张伟[1] 刘伯齐[1] 王洪平[1] 韩志楷[1] 韩双廷 曲平[1] 李茉[1] 丁镇伟[1] 林培中[1] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤研究所肿瘤医院肿瘤生物检测中心,北京100021

出　　处：《中华肿瘤杂志》2001年第2期129-131,共3页Chinese Journal of Oncology

基　　金：国家"九五"肿瘤攻关资助项目! ( 96 90 6 0 1 0 2 ) ;国家自然基金!资助项目 ( 3 9870 83 8)

摘　　要：目的　研究食管癌高发区癌前病变及癌活检组织标本中Ki6 7、p5 3蛋白的异常表达 ,探讨其与食管癌变的关系及作为早期癌变生物学标志物的可能性。方法　对来自食管癌高发区河北磁县的正常食管黏膜组织和轻度、中度、重度不典型增生上皮以及原位癌活检组织共 36 6例 ,应用免疫组化技术对食管癌变过程中Ki6 7、p5 3蛋白的异常表达进行研究。结果　在正常黏膜、轻度、中度、重度不典型增生及原位癌组织中 ,Ki6 7异常表达检出率分别为 0 (0 / 2 5 )、40 5 % (30 / 74)、6 1 3% (6 5 /10 6 )、76 5 % (39/ 5 1)和 90 0 % (72 / 80 ) ,其中异常表达程度在中度以上者分别占 0 (0 / 2 5 )、2 7% (2 / 74)、11 2 % (12 / 10 6 )、41 2 % (2 1/ 5 1)和 5 8 8% (47/ 80 ) ;p5 3蛋白异常表达的阳性率分别为 4% (1/ 2 5 )、39 1% (2 7/ 6 9)、5 7 5 % (6 1/ 10 6 )、5 2 9% (2 7/ 5 1)和 6 7 9% (5 3/ 78) ,其中异常表达程度在中度以上者分别占 0 (0 / 2 5 )、10 1% (7/ 6 9)、2 4 5 % (2 6 / 10 6 )、39 2 % (2 0 / 5 1)和 48 7% (38/ 78)。p5 3蛋白及Ki6 7在正常黏膜中的表达 ,与不典型增生总体及原位癌组织的差异均有显著性 (P <0 0 0 1)。等级相关分析结果显示 ,p5 3、Ki6Objective To investigate alterations in expression of Ki67, p53 and iNOS proteins in esophageal carcinogenesis. Methods The expression of Ki67,p53 and iNOS proteins was detected by immunohistochemical staining of 366 endoscopic biopsy specimens of the esophagus collected from high incidence area of esophageal cancer in China. If the intensity of staining was scored as≥++, it was regarded as overexpression. Results The overespression rate of Ki67 was 0 for normal epithelium(NE), 2.7% for mild dysplasia (MD), 11.2% for moderate dysplasia (MoD), 412% for severe dysplasia (SD),and 588% for carcinoma insitu(CIS),respectively. That of p53 and iNOS proteins was 0 and 0 for NE,101% and 40% for MD, 245% and 75% for MoD,392% and 25% for SD,487% and 14% for CIS, respectively. There was significant difference in the expression of Ki67 and p53 between normal epithelium and dysplasia of various degrees and CIS.Overexpression of Ki67 and p53 correlated well with the pathological grading of the lesions. Positive correlation was also found between p53 and Ki67 overexpressions. Conclusion Overexpression of Ki67 and p53, but not iNOS, is associated with carcinogenesis of the esophagus. They are early events in esophageal carcinogenesis and useful biomarkers for early detection.

关 键 词：食管癌 癌前状态 KI67 P53 INOS 免疫组织化学 

分 类 号：R735.1[医药卫生—肿瘤] R730.231[医药卫生—临床医学]