ATRA和雄黄对白血病细胞PML基因及蛋白表达的影响  

Study of Expression of PML Gene and PML Protein in Leukemia Cells

作　　者：钟璐[1] 陈芳源[1] 韩洁英[1] 邵念贤[1] 欧阳仁荣[1] 

机构地区：[1]上海第二医科大学仁济医院血液科

出　　处：《上海第二医科大学学报》2001年第2期106-109,133,共5页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金资助课题 (36970 32 8)

摘　　要：目的探讨PML基因及蛋白在白血病细胞中的表达和细胞内分布定位。　方法经ATRA、雄黄处理后的NB4、HL - 60、K5 62细胞为研究对象 ,细胞形态学观察采用Wright s染色法及荧光染色法 ;PML蛋白细胞内分布定位采用免疫荧光技术 ;PMLmRNA表达采用RT -PCR法。　结果 1 ATRA处理后 ,NB4和HL - 60细胞形态学上出现分化表现 ,K5 62细胞无变化 ;经雄黄处理后 ,各组细胞形态学上均出现凋亡特征性改变。 2 ATRA处理后 ,NB4细胞内融合蛋白降解 ,PML蛋白恢复定位 ,HL - 60、K5 62细胞内PML蛋白定位分布无变化 ;雄黄处理后NB4细胞内融合蛋白降解 ,PML聚积于POD结构 ,随后降解 ;在HL - 60及K5 62细胞 ,PML发生相似改变。3 ATRA、雄黄处理后各组细胞PMLmRNA表达均无显著影响。　结论在不同诱导剂刺激下 ,PML基因在蛋白水平参与细胞终末分化及诱导白血病细胞凋亡机制 ;PML蛋白在POD中发挥诱导凋亡控制细胞生长的作用。Objective To investigate the PML gene and protein expression and localization in leukemia cell lines. Cell morphology was assayed by Wrights stain, fluorescence stain, and PMLmRNA expression by RT-PCR, PML protein localization by immuno-fluorescence. NB4 and HL-60 cells differentiated morphologically after treatment with ATRA while K562 cells did not differentiate. Typical apoptosis was found in each cell line after treatment with red orpiment. Immuno-fluorescence analysis showed after treatment with ATRA fusion protein disappeared in NB4 cells and PML protein because relocated, while HL-60 and K562 cells had no difference from control cells. After treatment with red orpiment, fusion protein was disappeared in NB4 cells, then degraded, there also seen in HL-60 cells and K562 cells. The expression of PMLmRNA was not changed in all three cell lines after treatment with ATRA or red orpiment. PML plays the role of differentiation and apoptosis inducer in leukemia cells at the translational level; in POD PML acts on apoptosis and growth of leukemia cells.

关 键 词：PML ATRA 雄黄 NB4细胞 HL-60细胞 白血病 细胞凋亡 基因表达 

分 类 号：R733.7[医药卫生—肿瘤]