HPLC-DAD法同时测定脑健胶囊中4种指标成分含量  

HPLC-DAD Simultaneous Determination of Four Components in Naojian Capsules

作　　者：刘芳[1] 王宇红[2] 蔡川[2] 向韵[2] 蔡光先[2] 王籽又 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南中医药大学中药粉体关键技术及装备国家地方联合工程实验室,湖南长沙410208

出　　处：《中医学报》2014年第6期772-775,共4页Acta Chinese Medicine

基　　金：国家重点基础研究发展计划(编号:2012CB723503)

摘　　要：目的：建立脑健胶囊HPLC-DAD指纹图谱,并对其指标成分川芎嗪、阿魏酸、洋川芎内酯A、藁本内酯进行含量测定。方法：采用Elipse XDB-C18（250 mm×4.6 mm,5μm）色谱柱,柱温30℃,以0.3%乙酸水和乙腈为流动相进行梯度洗脱,流速1.0 mL·min-1,采用DAD检测器,检测波长为川芎嗪295 nm,阿魏酸325 nm,洋川芎内酯A 280 nm,藁本内酯328 nm。结果：脑健胶囊中4种指标成分能实现同时分离,川芎嗪、阿魏酸、洋川芎内酯A、藁本内酯的线性范围分别为：0.1033~2.066 g（R2=0.9999）,0.04966~1.9864 g（R2=0.9995）,0.1011~2.022 g（R2=1）,0.4072~2.066 g（R2=1）;平均加样回收率分别为95.60%（RSD 1.50%）,97.44%（RSD 1.63%）,106.2%（RSD 0.25%）,99.36%（RSD 0.71%）。结论：该方法客观可靠,适用于脑健胶囊全成分分析,实现综合质量控制。Objective ：To establish an HPLC-DAD method for acquiring fingerprint of Naojian capsules, also simuhaneous determination of ligustrazine,ferulaic acid, senkyunilide A and ligustilide. Methods：The analysis was carried out on Elipse XDB-C18 （250 mm× 4.6 mm,5 μm） column with acetonitrile- 0.3% acetic acid solution as mobile phase for gradient elution, the column temperature was maintained at 30℃ ;The flow rate was 1.0 ml/min;The detection wavelength was set at 295 nm,325 nm ,280 nm,328 nm separately. Results ：The four compounds in their respective peak areas had good lineatities （ R^2 ≥ 0. 999 5 ） and the average recoveries were 95.60% - 106.2%, RSD was within 0.25% - 1.63%. Conclusion：This method is objective and reliable, which is suitable for total components analysis and evaluation the overall quality of Naojian capsules.

关 键 词：脑健胶囊 HPLC-DAD法 川芎嗪 阿魏酸 洋川芎内酯A 藁本内酯 

分 类 号：R284.2[医药卫生—中药学]