低频电刺激脚桥被盖核对帕金森病大鼠步态和运动能力的影响  

Effect of low frequency stimulation in the pedunculopontine tegmental nucleus on gait and motor activity in hemiparkiusonisom rat models

作　　者：杨勇[1] 张旺明[1] 郑伟新[1] 罗敏捷[1] 王军[1] 周铭[1] 文朋 

机构地区：[1]南方医科大学珠江医院神经外科,广东神经外科研究所,广东省脑功能修复与再生重点实验室,广州510280

出　　处：《中华神经医学杂志》2014年第6期552-557,共6页Chinese Journal of Neuromedicine

基　　金：国家自然科学基金（30872662、81271250）;广东省科技计划引导项目（2008A03102010）;国家临床重点建设专科资助项目

摘　　要：目的研究低频电刺激脚桥被盖核（PPTg）对偏侧帕金森病（PD）大鼠步态及运动能力的影响。方法32只雄性SD大鼠按随机数字表法分为对照组（n=8）和实验组（n=24）；2组大鼠采用立体定向手术将6-羟基多巴胺注入大鼠右侧前脑内侧束（MFB），实验组大鼠在此基础上将电极植入右侧PPTg。分别于立体定向术前1周、术后3周对2组大鼠行Catwalk步态及旷场实验并采集数据；术后4周．实验组行低频电刺激PPTg后再次采集行为学数据。结果Catwalk步态实验结果显示，与术前比较，2组大鼠术后的双爪耦合值（右前→左前、右前→左后、右后→左前、右后→左后）均增加，术后速度变化百分比均增加，每秒步数均减少，差异有统计学意义（P〈0．05）。与刺激前比较，实验组大鼠低频电刺激PPTg后双爪耦合值均减少，速度变化百分比减少，每秒步数增加，差异有统计学意义（P〈0．05）。旷场实验中：实验组和对照组大鼠术后运动距离明显减少。实验组大鼠行低频电刺激PPTg后运动距离较刺激前明显增加，差异均有统计学意义（P〈0．05）。结论低频电刺激PPTg可改善偏侧PD大鼠步态和运动能力，Catwalk步态实验及旷场实验可有效检测该刺激作用的效果。Objective To study the effect of low frequency stimulation （LFS） in the pedunculopontine tegrnental nucleus （PPTg） on gait and movement ability in unilateral lesioned Parkinson＇s disease（PD） rats. Methods Thirty-two male SD rats were randomly assigned to control group （6-hydroxydopamine treatment, n=8） and experimental group （6-hydroxydopamine treatment combined with electrode implantation, n=24）. All subjects were processed with injection of 6-hydroxydopamine in the right medial forebrain bundle （MFB） and simultaneously the rats in the experimental group were received an electrode implantation into the ipsilateral PPTg. Catwalk gait and open field test data were acquired at 1 week before stereotactic surgery and 3 weeks after operation in the rats of control and experimental groups; the behavioral data of rats in the experimental group were recorded again 4 weeks after operation when they were under ongoing LFS-PPTg. Results Catwalk results showed that the postoperative couplings values （right front→left front, right front→left hind, right hind→left front, right hind→left hind） were significantly increased in all subjects as compared with the preoperative ones （P〈0.05）; while couplings value in rats of the experimental group after LFS-PPTg significantly reduced as compared with that before LFS-PPTg （P〈0.05）. The postoperative run speed variation statistically increased and postoperative cadence significantly decreased in all rats as compared with the preoperative ones （P〈0.05）; the run speed variation significantly decreased and cadence obviously increased in experimental group after LFS-PPTg as compared with those before LFS-PPTg （P〈0.05）. Open field test： the moving distance of all subjects decreased significantly after operation; the distance in rats of the experimental group after LFS-PPTg increased significantly as compared with that before LFS-PPTg. Conclusion LFS-PPTg can effectively improve gait function and motor activity of unilateral PD r

关 键 词：脚桥被盖核 低频电刺激 帕金森病 Catwalk步态系统 旷场实验 

分 类 号：R742.5[医药卫生—神经病学与精神病学]