藤茶双氢杨梅树皮素对人视网膜母细胞瘤HXO-RB_(44)细胞凋亡的诱导作用  

Ampelopsin induces apoptosis of human retinoblastoma HXO-RB_(44) cells

作　　者：唐琪[1] 郑作文[2] 蒋林志[3] 严宇清[3] 何剑峰[3] 梁皓[3] 

机构地区：[1]南宁市第一人民医院眼科,广西南宁530000 [2]广西中医学院药学院,广西南宁530000 [3]广西医科大学第一附属医院眼科,广西南宁530021

出　　处：《实用肿瘤杂志》2014年第3期223-227,共5页Journal of Practical Oncology

基　　金：广西自然科学项目资助(2011GXNSFA018243);广西中医药管理局项目(gzzc1052)

摘　　要：目的观察中药单体成分双氢杨梅树皮素(ampelopsin,APS)对人视网膜母细胞瘤(retinoblastoma,RB)细胞株HXO-RB44凋亡的影响,以探讨中药治疗RB的可能性。方法取对数生长期的人视网膜母细胞瘤细胞株HXO-RB44用于实验,不同浓度的APS作用于HXO-RB44细胞24小时后,以MTT法、生长曲线法、软琼脂克隆形成法观察APS对HXO-RB44的体外抑制作用;倒置显微镜观察其形态学变化;AO/EB染色观察细胞凋亡形态学变化;流式细胞仪检测细胞凋亡。结果 MTT法检测证实,APS对HXO-RB44的IC50为14.71 mg/L;细胞生长曲线法提示其对HXO-RB44细胞生长有明显抑制作用;软琼脂克隆形成法观察APS浓度>9.90 mg/L时抑制率为100.0%,药物浓度为4.40 mg/L,抑制率为52.2%。APS作用后出现了明显的凋亡细胞,表现为细胞皱缩、核染色质浓缩、核碎裂等凋亡特征性的形态学改变。流式细胞仪检测50.00 mg/L、14.84 mg/L、4.40 mg/L和0.00 mg/L APS作用24小时后的细胞凋亡率分别为88.1%、58.5%、14.8%和4.8%,各浓度组凋亡率与空白对照组(4.8%)比较,差异均有统计学意义(P<0.05),各浓度组细胞凋亡率之间差异也均有统计学意义(P<0.05)。结论 APS对HXO-RB44细胞的体外增殖活性有明显的抑制作用,并可诱导体外培养的RB细胞凋亡,可作为中药治疗RB的候选药物。Objective To investigate the effect of ampelopsin on the apoptosis of human retinoblastoma HXO-RB44 cells. Methods Human retinoblastoma HXO-RB44 cells were treated with different concentration of ampelopsin in vitro for 24 h. Cell proliferation was measured by MTT and cell colony formation test. Morphological changes were observed under optical microscopy. Cell apoptosis was observed by AO/EB staining and flow cytometry. Results MTT assay showed that ampelopsin significantly inhibited the growth of cultured HXO-RB44 cells with an IC50 of 14.71 mg/L. Cell colony test revealed that the repressive rate was 100.0% and 52. 2% after treatment with 9.90 mg/L and 4.40 mg/L ampelopsin, respectively. AO/EB fluorescent staining showed typical morphological changes of cell apoptosis after the drug treatment, including cell shrinkage ,chromatin condensation and nuclear fragmentation. The apoptosis rates were 88. 1% , 58. 5% , 14. 8% and 4. 8% after 24 h treatment with 50. 00 mg/L, 14. 84 mg/L,4. 40 mg/L and 0.00 mg/L of ampelopsin （ P 〈 0. 05 ）. Conclusion Ampelopsin can inhibit the growth and induce the apoptosis of human retinoblastoma HXO-RB44 cells in vitro.

关 键 词：视网膜肿瘤 病理学 杨梅素 药理学 黄酮醇类 药理学 茶 化学 视网膜母细胞瘤 药物疗法 视网膜母细胞瘤 病理学 细胞凋亡 药物作用 细胞系 肿瘤 

分 类 号：R739.7[医药卫生—肿瘤] R730.2[医药卫生—临床医学]