最佳剂量链脲佐菌素构建糖尿病小鼠的淋巴瘤模型  

Optimization of streptozotocin dosing for establishing tumor-bearing diabetic mouse models

作　　者：汤瑶[1,2] 雷翔慧 简文静[2] 严金海[2] 吴自勍[1,2] 赵彤[1,2] 

机构地区：[1]南方医科大学南方医院病理科,广东广州510515 [2]南方医科大学基础医学院病理系,广东广州510515

出　　处：《南方医科大学学报》2014年第6期827-831,共5页Journal of Southern Medical University

基　　金：国家自然科学基金(81071659)~~

摘　　要：目的选择合适剂量的链脲佐菌素(STZ)诱导形成糖尿病鼠,而后用该鼠构建小鼠淋巴瘤的荷瘤模型。方法健康雄性Balb/c小鼠200只随机分为正常对照组(50只)和实验组(75、150、200 mg/kg d STZ,各50只)。一次性腹腔注射不同剂量STZ,定期监测小鼠体质量和血糖变化,观察各组造模成功率及死亡率,生存时间。糖尿病造模成功后皮下或尾静脉注射A20淋巴瘤细胞,分别在1个月后、3个月后观察成瘤率、死亡率,生存时间。结果 STZ 150 mg/kg组和STZ 200 mg/kg组与对照组相比,体重均显著降低,血糖显著升高(均P<0.05),而STZ 75 mg/kg组改变不明显。STZ 200 mg/kg组死亡率明显高于STZ 150 mg/kg组,生存时间明显缩短(均P<0.05)。正常对照组、STZ 150 mg/kg组、STZ 200 mg/kg组皮下注射A20淋巴瘤细胞死亡率、成瘤率无统计学差异(P>0.05),尾静脉注射3个月后有统计学意义(P<0.05)。结论一次性腹腔注射STZ 150 mg/kg死亡率低,糖尿病成模率高,生存时间长,是构建Balb/c小鼠移植瘤模型的理想剂量。Objective To determine the optimal dosing of streptozotocin （STZ） for establishing lymphoma-bearing diabetic mouse models. Methods A total of 200 healthy male Balb/c mice were randomized into 4 groups （n=50） for intraperitoneal injection of a single dose of vehicle solution （control） or 75, 150, or 200 mg/kg STZ. The changes of body weight and blood glucose were observed regularly, and the success rate of modeling, mortality rate, and survival of the mice were recorded after the injections. The mice with successfully induced diabetes received subcutaneous or tail vein injection of A20 lymphoma cells, and the rate of tumorigenesis, mortality rate, and survival time were observed at 1 month and 3 months after tumor cell injection. Results Compared with the control group, the mice receiving STZ injection at 150 and 200 mg/kg showed significantly decreased body weight and increased blood glucose （P〈0.05）, while STZ at 75 mg/kg did not produced such obvious changes. STZ injection at 200 mg/kg resulted in a significantly higher mortality rate and shorter survival time than STZ at 150 mg/kg （P〈0.05）. In the control group and 150 and 200 mg/kg STZ groups, the rate of tumorigenesis or mortality rate showed no significant differences after subcutaneous injection of A20 lymphoma cells （P〉0.05）, but differed significantly at 3 months after tail vein injection of the tumor cells （P〈0.05）. Conclusion Intraperitoneal injection of STZ at 150 mg/kg is associated with a low mortality rate, a high successful modeling rate of diabetes and a long survival time in mice, and is therefore optimal for establishing diabetic mouse models bearing transplanted tumors.

关 键 词：链脲佐菌素 糖尿病 模型 BALB C小鼠 淋巴瘤 

分 类 号：R-332[医药卫生]