miR-200c通过wnt/β-catenin信号途径抑制SW480结肠癌细胞的侵袭和转移  

MiR-200c inhibits proliferative and invasive ability of human colon cancer cell in vitro through Wnt/β-catenin signaling pathway

作　　者：武健[1] 吴莺[1] 孟凡鲁[1] 贺明洁[1] 袁英雪 周红[2] 王文兵[3] 

机构地区：[1]江苏大学附属医院消化病科,212001 [2]江苏大学医学检验系 [3]江苏大学生命科学院

出　　处：《江苏医药》2014年第11期1260-1262,F0003,共4页Jiangsu Medical Journal

基　　金：江苏省自然科学基金(BK2010336)

摘　　要：目的研究miR-200c通过wnt/β-catenin信号通路对结肠癌SW480细胞侵袭、迁移的影响。方法将SW480细胞分为类似物转染(A)组、抑制物转染(B)组、类似物阴性对照(C)组、抑制物阴性对照组(D)和空白(E)组。荧光定量PCR检测12h和24h转染效率,划痕实验和Transwell侵袭实验检测细胞迁移和侵袭,Western blot检测转染后β-catenin和E-cadherin的表达。结果细胞划痕实验显示,A组12h和24h后伤痕宽度分别为(589.61±17.28)μm和(523.83±57.13)μm,C组分别为(465.33±16.60)μm和(393.99±7.53)μm,A组高于C组(P<0.05)。B组12h和24h后分别为(430.93±20.76)μm和(221.38±44.37)μm,D组分别为(485.64±16.65)μm和(441.22±22.40)μm,B组低于D组(P<0.05)。Transwell实验显示,与C组和D组相比,A组24h和48h通过8μm孔径的细胞数明显减少,B组明显增多(P<0.05)。A组β-catenin和E-cadherin的表达均升高,B组各蛋白表达下降(P<0.05)。结论 miR-200c可能通过wnt/β-catenin信号通路抑制SW480结肠癌细胞的侵袭和迁移。Objective To explore the effect of miR-200c on the proliferative and invasive activities of colon cancer cell line SW480 through wnt/β-eatenin signaling pathway. Methods SW480 cells were divided into 4 groups of A（transfected with analogue）, B（transfected with inhibitor）, C （analogue negative control）, D（inhibitor negative control） and E（blank control）. The transfection efficiency was detected by RT-PCR. The cells migration and invasion were detected by scratch test and transwell experiment. The expressions of β-catenin and E-cadherin were detected by Western blot. Results The scar width in group A was （589.61±17.28）μm at 12 h and （523.83±57. 13）μm at 24 h,which in group C was （430. 93±20. 76）μm and （221.38±4-44.37）μm, in group B was （465.33 ±16.60）μm and （393.99±7. 53）μm,in group D was （485.64±16.65）μm （441.22±22.40）μm （P〈0. 05）. Compared with groups C and D at 24 h and 48 h,the number of the cells passed 8μm hole was less in group A and more in group B（P〈0. 05）. The expressions of β-catenin and E-cadherin in group A were increased, which were decreased in group B（P〈0. 05）. Conclusion miR-200c may inhibit the abilities of migration and invasion of SW480 cells through wnt/β-catenin signaling pathway.

关 键 词：MIR-200C WNT Β-CATENIN信号通路 结肠癌SW480细胞 

分 类 号：R735[医药卫生—肿瘤]