GPC3与肝癌细胞增殖的相关性  

Correlation between GPC3 expression and proliferation of hepatocellular carcinoma cells

作　　者：李海荣[1] 黄兰姗 陈罡[1] 陆会平[1] 冯振博[1] 

机构地区：[1]广西医科大学第一附属医院病理科,广西壮族自治区南宁市530021

出　　处：《世界华人消化杂志》2014年第15期2108-2115,共8页World Chinese Journal of Digestology

基　　金：广西科技攻关基金资助项目;Nos.10124001A-1;1298003-2-5~~

摘　　要：目的:探讨肝细胞癌(hepatocellular carcinoma,HCC)组织内及体外细胞培养中磷脂酰肌醇蛋白聚糖-3(glypican-3,GPC3)与细胞增殖的关系.方法:采用免疫组织化学方法检测54例HCC组织中GPC3的表达,并对比其与Ki-67标记指数及各种临床病理参数的关系.使用shRNA抑制SMMC7721肝癌细胞系中GPC3的表达,MTT检测肝癌细胞的增殖变化.结果:在HCC组织中,GPC3与Ki-67指数有关(r=0.521,P=0.000),也与分化程度、血管浸润情况具有相关性(r=0.287,0.289;P=0.035,0.034).体外实验结果显示,抑制GPC3表达后,实验组细胞增殖活性明显低于阴性对照组和空白对照组(F=45.38,P=0.000).结论:GPC3在HCC的发生、发展和浸润过程中起重要作用,主要与影响肝癌细胞增殖有关.AIM： To investigate the relationship between GPC3 expression and proliferative ability of hepatocellular carcinoma （HCC） cells. METHODS： Immunohistochemistry was employed to detect the expression of GPC3 in 54 HCC tissues. The correlation between GPC3 expression and Ki-67 labeling index （Li） and other clinicopathologic characteristics was studied. An shRNA targeting the GPC3 gene was transfected into SMMC7721 cells to silence GPC3 expression. MTT assay was performed to determinethe effect of GPC3 knockdown on the growth of HCC SMMC7721 cells. RESULTS： GPC3 expression was significantly correlated with Ki-67 Li （r = 0.521, P = 0.000）. GPC3 expression was related to differentiation degree and vessel invasion of HCC （r = 0.287, 0.289; P = 0.035, 0.034）. The cell proliferative activity was significantly reduced in the GPC3- shRNA group compared with the negative control group and blank control group （F = 45.38, P = 0.000）. CONCLUSION： GPC3 may play an important role in the occurrence, development and invasion of HCC, mainly due to the effect on the proliferation of HCC cells.

关 键 词：磷脂酰肌醇蛋白聚糖-3 KI-67 肝癌细胞 增殖 

分 类 号：R735.7[医药卫生—肿瘤]