ERK1/2通路对Genistein与5-FU诱导的人肝癌细胞周期阻滞的影响  被引量:4

Role of ERK1/2 pathway in genistein and 5-FU-induced cell cycle arrest in human hepatoma cells

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作  者:刘丹[1] 赵忠新 迟大鹏[3] 梅庆步[1] 于海涛[1] 岳丽玲[1] 王玉[1] 陈萍[1] 郑立红[1] 王秀华[1] 王钰粟 

机构地区:[1]齐齐哈尔医学院基础医学院,黑龙江省齐齐哈尔市161006 [2]齐齐哈尔市建华医院普外科,黑龙江省齐齐哈尔市161006 [3]上海中医药大学附属岳阳医院神经外科,上海市200437

出  处:《世界华人消化杂志》2014年第15期2134-2139,共6页World Chinese Journal of Digestology

基  金:黑龙江省教育厅科学技术研究基金资助项目;No.1253179;齐齐哈尔医学院科研基金资助项目;黑龙江省教育厅大学生创新训练基金资助项目;No.201311230026~~

摘  要:目的:探讨染料木黄酮(Genistein,Gen)与5氟尿嘧啶(5-fluorouracil,5-FU)诱导人肝癌M H C C97-L细胞周期阻滞与细胞外调节蛋白激酶(extracellular regulated protein kinasesERK)1/2信号通路的关系.方法:MTS法检测细胞增殖;流式细胞术检测细胞周期;Western blot检测总ERK1/2和磷酸化ERK1/2蛋白表达.结果:Gen与5-FU单用及联用均能抑制肝癌MHCC97-L细胞增殖;Gen诱导细胞S期阻滞少量抑制ERK1/2磷酸化,ERK1/2抑制剂可促进Gen对MHCC97-L细胞的生长抑制作用,而对S期阻滞无明显影响;5-FU单用和二者联用组均阻滞S期细胞,并显著激活ERK1/2磷酸化ERK1/2抑制剂可促进两组药物对MHCC97-L细胞的生长抑制和诱导S期阻滞作用.结论:Gen与5-FU单用及联用均可通过阻滞细胞周期来发挥抗肝癌细胞增殖的作用;抑制ERK1/2通路可抵抗Gen诱导的肝癌细胞S期阻滞,而促进5-FU单用和二者联用组诱导肝癌细胞S期阻滞.AIM: To investigate the role of the extracellular regulated protein kinases (ERK)I/2 pathway in genistein and 5-fluorouracil (5-FU)-induced cell cycle arrest in human hepatoma cells. METHODS: The MTS method was used to assay cell proliferation. Cell cycle was detected by flow cytometry. The protein expression of total ERK1/2 and phospho-ERK1/2 was detected by Western blot. RESULTS: Genistein and 5-FU, alone or in combination, inhibited proliferation of MHCC97-L cells. Genistein induced an S-phase arrest and slightly inhibited the expression of phospho- ERK1/2. ERK1/2 inhibitor could promote the inhibition of MHCC97-L cells by genistein, but had no significant effect on S-phase arrest. 5-FU alone or in combination with genistein arrested the cells in S phase and significantly activated ERK1/2. ERK1/2 inhibitor increased genistein and 5-FU-induced growth inhibition of MHCC97-L cells and S-phase arrest. CONCLUSION: Genistein and 5-FU inhibit the proliferation of liver cancer cells by arresting the cell cycle. Inhibition of the ERK1/2 pathway can resist genistein-induced S phase arrest in liver cancer cells, but promote S phase arrest induced by 5-FU alone or in combination with genistein.

关 键 词:细胞外信号调节激酶 染料木黄酮 氟尿嘧啶 肝癌 细胞周期 

分 类 号:R735.7[医药卫生—肿瘤]

 

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