荧光定量PCR法在胃癌血清miR-21检测中的应用  

Clinical significance of quantitative PCR detection of serum miR-21 in patients with gastric cancer

作　　者：朱伦 杨少芳 余算 郑阿迈 徐荣伟 

机构地区：[1]浙江省乐清市第三人民医院检验科,浙江省乐清市325600

出　　处：《世界华人消化杂志》2014年第15期2155-2158,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨miR-21对胃癌无创性诊断以及对手术效果的评判.方法:选取5类人员胃肠消化系统样本血清,通过荧光定量PCR方法对miR-21表达情况进行检测,对照分析正常体检人员和胃窦溃疡患者、胃窦炎性息肉患者、肠上皮化生人员以及胃癌患者的血清样本中分别与配对的胃组织miR-21表达情况.结果:通过对照分析,不同人员的血清中miR-21含量分别和配对的胃肠组织中表达的量具有正相关性(r=0.826,P<0.001).经过检测,和正常对照组进行比较,miR-21在胃癌患者的血清中含量出现明显升高,且术后,血清中miR-21的含量和手术之前比较降低明显(t=0.511,P=0.030).另外,miR-21在五组研究对象的血清中含量具有明显差异,具有统计学意义(χ2=10.841,P=0.028).结论:通过分析,利用检测血清中miR-21含量变化,可以无创性的诊断胃癌,并通过miR-21含量变化对手术的效果进行评价,临床上具有重要意义.AIM： To explore the significance of serum miR-21 detection for non-invasive diagnosis of gastric cancer and evaluation of surgery results. METHODS： Serum samples from normal medi- cal personnel, patients with antral ulcers, those with gastric inflammatory polyps, those with intestinal metaplasia, and those with gastric cancer were collected, and fluorescence quantitative PCR was used to detect miR-21 expression in the above serum samples and matched gastric tissues RESULTS： Serum miR-21 levels had a positive correlation with those in gastric tissues in all the subjects （r = 0.826, P 〈 0.001）. Serum levels of miR-21 were significantly higher in patients with gastric cancer than in normal controls. Postoperative serum levels of miR-21 were significantly lower than preoperative levels in gastric cancerpatients （t = 0.511, P = 0.030）. Serum miR-21 levels differed significantly among the five groups of subjects （2 = 10.841, P = 0.028）. CONCLUSION： Detection of serum miR-21 is helpful for noninvasive diagnosis of gastric cancer and evaluation of surgical effects.

关 键 词：荧光定量PCR法 胃癌 MIR-21 无创性诊断 

分 类 号：R735.2[医药卫生—肿瘤]