白细胞介素-24对喉癌细胞的增殖抑制效应及机制  

The inhibitory effect of interleukin-24on the proliferation of laryngeal cancer cells and its mechanism

作　　者：王睿[1] 王郡甫[2] 刘娣[3] 苏庆红[2] 周鹏[2] 陈雪梅[4] 许晓群[2] 

机构地区：[1]菏泽市立医院神经外科,山东菏泽274000 [2]山东省医学科学院基础医学研究所 [3]滕州市中心人民医院检验科 [4]山东大学第二医院耳鼻咽喉科

出　　处：《临床耳鼻咽喉头颈外科杂志》2014年第12期902-907,共6页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：山东省优秀中青年科学家科研奖励基金(No:BS2009SW007);山东省自然科学基金(No:ZR2010CM067;ZR2009CM036;ZR2012HL23);山东省大型科学仪器升级改造技术研究专项(No:2013SJGZ09)

摘　　要：目的:研究腺病毒介导的人白细胞介素-24(Ad-mda-7/IL-24)基因对喉癌细胞Hep-2增殖抑制效应及机制。方法:将携带有hIL-24基因的重组复制缺陷型腺病毒(Ad-hIL-24)感染喉癌细胞Hep-2,并以正常人脐静脉内皮细胞HUVEC为对照,采用RT-PCR方法检测Hep-2、HUVEC细胞中外源性hIL-24表达,以及Bcl-2和Bax的变化;采用四甲基偶氮唑蓝(MTT)比色法检测细胞的生长和凋亡情况。结果:腺病毒介导的hIL-24mRNA能在Hep-2细胞和HUVEC细胞中表达;在Hep-2细胞中,抗凋亡分子Bcl-2表达降低,而促凋亡分子Bax表达增强;在HUVEC细胞中Bcl-2表达没有变化,Bax表达有所增强。MTT法显示Ad-hIL-24能明显抑制喉癌细胞Hep-2的生长。在Hep-2细胞中,显微镜下可见明显的细胞凋亡。结论:Ad-hIL-24能抑制喉癌细胞Hep-2生长和诱导Hep-2细胞凋亡,但对正常细胞无毒性作用。Objective：To study the inhibitory effect of adenovirus mediated interleukin-24 gene on the proliferation of laryngeal cancer Hep-2 cells and its mechanism. Method：The human IL-24 gene was transfeeted into human laryngeal cancer cell line Hep-2 and human umbilical vein endothelial cell （HUVEC） with a replication-incompetent adenovirus vector. The hIL-24 gene expression was confirmed by RT-PCR in both Hep-2 cells and HUVEC cells. The expression of Bcl-2 and Bax were detected by RT-PCR and Western blot. The cell proliferation and the apoptotic effect were tested by MTT assay and fluorescence microscopy. Result： The expression of hIL-24 were confirmed in Hep-2 cells and HUVEC cells by RT-PCR. RT-PCR and Western blot showed the expression of Bcl- 2 significantly decreased in Hep-2 cells transfected with Ad-hIL-24 but not in transfected HUVEC cells, while the expression of Bax was increased in both transfected cells. MTT assay indicated that Ad-hIL-24 could induce proliferation inhibition in Hep-2 cells but not in HUVEC cells. Obvious apoptosis wan observed in Hep-2 cells under the microscope. Conclusion： Ad-hIL-24 can selectively inhibit proliferation and induces apoptosis of Hep-2 cells. However, it has no toxic effects on normal cells.

关 键 词：白细胞介素-24 腺病毒 喉癌细胞 凋亡 

分 类 号：R739.65[医药卫生—肿瘤]