海洋假交替单胞菌抗真菌活性物质的纯化和鉴定  

Purification and characterization of antifungal material produced by marine Pseudoalteromonas ruthenica

作　　者：梁静娟[1] 庞宗文[1] 张琳[1] 戚启琼[1] 

机构地区：[1]广西大学生命科学与技术学院,广西南宁530005

出　　处：《广东农业科学》2014年第11期114-117,共4页Guangdong Agricultural Sciences

基　　金：广西科学基金(桂科自0832040)

摘　　要：海洋假交替单胞菌JIV-49产生的抗真菌活性物质对白色念珠菌、新型隐球菌、烟曲霉、黑曲霉、红色毛癣菌和须癣毛癣菌等多种真菌的生长有明显抑制作用。为了明确其抗真菌活性物质的化学本质,采用透析、活性炭脱色、丙酮-乙醇沉淀等方法对JIV-49发酵液中的抗真菌活性物质进行粗提取,得到了活性成分的粗提液。粗提液经AB-8大孔吸附树脂柱层析、Sephadex G-100柱层析和Sephadex G-25柱层析进一步纯化,经硅胶薄层层析检查证明抗真菌活性物质已得到纯化,得到海洋细菌JIV-49产抗真菌活性成分的纯化物。对纯化后的抗真菌活性物质进行茚三酮反应、紫外光谱和红外光谱分析,确定该活性物质为环脂肽。对该活性物质进行抗真菌谱分析,发现该物质的抗真菌谱比目前临床使用的抗真菌药物更广。The antifungal material produced by Pseudoalteromonas ruthenica JIV-49 significantly inhibited the growth of Candida albicans, Cryptococcus neoforman, Aspergillus fumigatus, A. niger, Trichopyhton rubrum and T. mentagrophytes. The antifungal material was preliminarily separated from the crude fermentation broth of JIV-49 by filtration through 8 kDa dialysis bag, deeolorization with charcoal and precipitation with acetone and ethanol. It was further purified by AB-8 maeroporous resin absorption chromatography, Sephadex G-100 and Sephadex G-25 gel filtration chromatography. The purified antifungal material showed a single spot on silica gel thin layer chromatography. The purified antifungal material was identified as a lipopeptide by ninhydrin reaction, UV spectrum analysis and infrared spectrum analysis. The inhibition to fungi of the purified antifungal material was detected. The results indicated that the antifungal spectrum of the purified antifungal material from JIV-49 was broader than those of the antifungal drugs commonly used in clinical.

关 键 词：海洋细菌 假交替单胞菌 抗真菌 纯化 鉴定 

分 类 号：Q939[生物学—微生物学]