花青素合成转录因子表达载体的构建及遗传转化油菜研究  

Study on construction of anthocyanin transcription factor expression vector and transformation into Brassica napus

作　　者：聂鑫[1] 陈国平[2] 张利[1] 李再新[1] 谢万如[1] 赵志平[1] 

机构地区：[1]四川理工学院化学与制药工程学院,四川自贡643000 [2]重庆大学生物工程学院,重庆400044

出　　处：《广东农业科学》2014年第11期149-153,F0003,共6页Guangdong Agricultural Sciences

基　　金：四川省教育厅自然科学基金(11ZB102);四川理工学院人才引进项目(2011RC12)

摘　　要：花青素是类黄酮化合物的衍生物,具有抗氧化功能。油菜是全世界广泛种植的油料作物,但其花青素含量低、抗氧化能力弱。为了利用金鱼草花青素生物合成转录因子遗传转化油菜提高其花青素含量,通过PCR从金鱼草cDNA中扩增了Roseal1和Delila基因,并分别克隆到含有35S启动子和35S终止子的pDH51载体。然后将含有35S启动子、基因片段和35S终止子的片段分别连接到pBIN19载体,获得了含有双35S启动子和终止子的表达载体pBIN-Delila-Roseal1。通过农杆菌介导遗传转化甘蓝型油菜后,获得了紫色的愈伤组织。研究结果为利用基因工程技术提高油菜花青素的含量奠定了基础。Anthocyanins are flavonoid derivatives and have good antioxidant capacity. Brassica napus is a kind of oil plant and widespread all over the world. However, anthocyanin level and antioxitant capacity of B. napus are low. And, little attention is paid to genetic transformation of snapdragon A ntirrhinum majus anthocyanin biosynthesis transcription factors into B. napus to enhance the anthocyanin content. In this study, Roseall and Delila gene fragment were amplified by PCR from snapdragon A. majus eDNA, respectively. Then, the two gene fragments were cloned into pDH51 including 35S promoter and 35S terminator respectively. Fragments containing 35S promoter, target gene and 35S terminator were ligated into pBIN19 respectively, resulting in the expression vector pBIN-Delila-Roseall containing double 35S promoter and terminator. The callus of the transformed B. napus turned out to be purple compared to that of the wild-type strain. The result provides basis for using genetic engineering technology to increase the anthocyanin levels of B. napus.

关 键 词：花青素 转录因子 表达载体 油菜 

分 类 号：S635.3[农业科学—蔬菜学] Q786[农业科学—园艺学]