培哚普利对糖尿病大鼠肾组织内p-smad2及Ⅳ型胶原表达的影响  

The effect of perindopril to p-smad2 and collagen Ⅳ in the kidney of diabetic rats

作　　者：尹代婵[1] 陈敏[1] 王星娜[1] 

机构地区：[1]长沙市第四医院内分泌科,湖南长沙410006

出　　处：《中国现代医学杂志》2014年第13期23-27,共5页China Journal of Modern Medicine

摘　　要：目的探讨血管紧张素转化酶抑制剂培哚普利对链脲佐菌素(STZ)诱导的糖尿病大鼠模型肾组织Smad2活化形式p-smad2及Ⅳ型胶原蛋白表达的影响。方法链脲佐菌素诱导的16只糖尿病大鼠模型,随机分为模型组和培哚普利治疗组,另以8只正常大鼠作为对照组。于24周末观察血糖、血压、24 h尿蛋白、肾重/体重。免疫组织化学检测肾组织p-smad2、Ⅳ型胶原蛋白表达。Western-blot检测大鼠肾组织p-smad2蛋白表达水平。结果模型组和治疗组血糖明显高于正常组,但治疗组和模型组差异无显著性,模型组尿白蛋白水平均显著高于正常组,治疗组尿蛋白较模型组显著降低。免疫组化半定量结果示p-smad2蛋白在培哚普利治疗组较模型组表达显著减少,Ⅳ型胶原在培哚普利治疗组较模型组表达显著减少。Western-blot示p-smad2在治疗组较模型组显著减少。结论培哚普利可以通过影响糖尿病大鼠肾脏内Smad2活化,从而下调Ⅳ型胶原表达来延缓糖尿病纤维化进程。It was the aim of our study to investigate the effect of perindopril on p-smad2 as well as collagenIV in the kidney of diabetic rats induced by streptozotocin（STZ）. 【Methods】Sixteen Sprague-Dawley rats injected with STZ were randomly divided into control（untreated） and treatment of perindopril group, other eight normal rats were used as control. At 24 weeks, the blood-sugar levels, blood pressure, urine protein of 24 hours, kidney/body ratio were detected, the expression of p-smad2 and collagenⅣ protein were also detected by immunohistochemical method while p-smad2 were examined by western blotting.【Results】The blood-sugar level in untreated and treated group was much less than normal group, but no differences between untreated and treated group, the urine protein in untreated group was much more than normal group, but there was a much decrease in treated group than untreated group. Immunohistochemical semiquantitative method indicated that there was a decrease in perindopril treated group in p-smad2 and collagen Ⅳ when compared with untreated group. Western-blot results also showed that p-smad2 in perindopril treated group was much less than untreated group（P 0.05）.【Conclusion】 Perindopril may be useful in preventing the fibrosis progression in the kidney of diabetic rats through influencing the activation of smad2 as well as decreasing collagenⅣ.

关 键 词：培哚普利 p-smad2 Ⅳ型胶原 糖尿病大鼠 

分 类 号：R-332[医药卫生]