BDA示踪观察脊髓胸腰段损伤后不同时间段膀胱排尿神经通路的改变  

BDA anterograde neuronal tracing in the spinal cord injury rat's micturition upstream transduction pathway in different period

作　　者：杨伟东[1] 双卫兵[1] 孟称心[2] 程涛[1] 

机构地区：[1]山西医科大学第一医院,山西太原030001 [2]山西省儿童医院,山西太原030013

出　　处：《中国现代医学杂志》2014年第15期15-19,共5页China Journal of Modern Medicine

基　　金：国家青年科学基金项目(No:30901483);山西省高校优秀青年学科带头人资助项目(晋教科[2012]10);山西省自然科学基金项目(No:2013011053-1);新疆生产建设兵团六师科技计划项目(No:1321);新疆生产建设兵团2013年卫生科技计划项目(兵卫发[2013]32号)

摘　　要：目的应用生物素葡聚糖胺（BDA）示踪技术观察大鼠背侧胸腰段脊髓损伤（SCI）后不同时间段膀胱排尿神经通路改变的差异。方法清洁级雌性SD大鼠90只，随机分为5组：1 d组、3 d组、7 d组、14 d组、21 d组（每组分别设对照组8只，SCI组10只）。使用标准化脊髓损伤动物模型实验装置制备背侧脊髓损伤动物模型，损伤部位定于T10－L1水平，对照组大鼠仅咬除棘突和椎板，不损伤脊髓。模型制备后分别于1 d、3 d、7 d、14 d、21 d给予对照组及SCI组大鼠膀胱壁注射BDA。注射2周后取材，免疫组化方法显示大鼠脊髓切片中BDA阳性神经元。结果BDA阳性神经元经免疫组化学方法显色后呈椋褐色。SCI 1 d、3 d、7 d、14 d、21 d各组脊髓T10-L1平面的阳性神经细胞数与对照组相比均明显减少，SCI 14 d、21 d组较SCI 3 d、7 d组阳性神经细胞数又有所增加。结论大鼠背侧胸腰段脊髓损伤后支配排尿的神经通路受损，在损伤早期呈现出损伤进行性加重趋势，至损伤后1周左右脊髓神经细胞出现修复，神经通路可有部分修复。[ Objective ] To observe the changes of dorsal spinal cord injury （SCI） rat＇s micturition upstream pathway by biotin dextran amine （BDA） anterograde neuronal tracing technique in different period. [ Methods ] 90 clean grade Female Sprague-Dawley （SD） rats were divided into 5 groups randomly. They are 1 day group, 3 days group, 7 days group, 14 days group and 21 days group. Each group has two sub-group, the control group has 8 animals, and dorsal spinal cord injury group has 10 animal. We prepared the dorsal spinal cord injury model by ourselves-designed device, and selected T10-L1 as the level of injury. In the control group, the spinal cord was identically exposed, and the spinal cord not damaged. According grouping the rats injected BDA into bladder wall at 1 day, 3days, 7days, 21days after the preparation of animal models,respectively, and two weeks after BDA injection, make spinal cord tissues to biopsy and observed. [Results] The BDA-positive neurons were stained brown by immunohistochemistry methods. Compared with the control group, we found that the number of BDA-positive neurons of surgical group significantly decreased. Compared with SCI3d and SCI7d group, the numbers of BDA- positive neurons had increased in SCI14d and SCI21d group. [Conclusions] Dorsal spinal cord injury can make the micturition upstream transduction pathway damage . In early stage,it was progressively aggravated. About one week later, the spinal cord nerve cells repaired, and neural pathways could had part of the repair.

关 键 词：脊髓损伤 生物素葡聚糖胺 动物模型 脊髓神经细胞 

分 类 号：R-332[医药卫生]