荷瘤小鼠骨髓来源的树突状细胞诱导产生的抗肿瘤免疫反应  

Antitumor Immunity Induced by Dendritic Cells from Marrow of Tumorbearing Mouse

作　　者：唐华[1] 罗红艳 银平章[1] 孔令非[1] 

机构地区：[1]河南省人民医院生物治疗中心,郑州450003 [2]中国生物制品总公司,北京100101

出　　处：《中国生物制品学杂志》2001年第1期7-9,共3页Chinese Journal of Biologicals

摘　　要：目的研究荷瘤小鼠骨髓来源的树突状细胞（ＤＣ）是否具有诱导机体产生抗肿瘤免疫反应的能力， 与正常小鼠骨髓来源的ＤＣ有无差异。方法于体外用ｍＧＭ－ＣＳＦ和ｍＩＬ－４分别从正常小鼠和荷Ｃ２６肿瘤的小鼠骨 髓细胞诱导产生ＤＣ，观察它们刺激同种Ｔ细胞、同基因Ｔ细胞增殖的能力，以及由二者免疫所诱导产生的ＣＴＬ的 杀伤活性。结果荷瘤小鼠骨髓来源的ＤＣ与正常小鼠骨髓来源的ＤＣ在刺激同种Ｔ细胞增殖、刺激同基因Ｔ细胞 增殖的能力和诱导产生ＣＴＬ的杀伤活性差异无显著意义。结论荷瘤小鼠骨髓来源的ＤＣ与正常鼠骨髓来源的 ＤＣ具有相似的诱导产生抗肿瘤免疫反应的能力，提示可以从荷瘤机体的ＤＣ前体诱导产生有抗瘤能力的ＤＣ，用于 抗肿瘤免疫治疗。Objective To investigate whether the dendritic cells(DCs)from the marrow of tumor bearing mouse has the potent capacity of inducing antitumor immunity.Methods DCs were induced from normal and tumor-bearing mouse marrow with mGM-CSF and mIL-4 in vitro respecitvely.The T cell stimulating activities and the capacities of inducing CTL activity of the DCs were detected and compared.Results No significant difference was observed in the T cell stimulating activities and the capacities of inducing CTL activity of them.Conclusion Antitumor immune response could be induced potently by DCs from tumor-bearing mice.

关 键 词：树突状细胞 荷瘤 CTL GM-CSF 抗肿瘤免疫反应 

分 类 号：R730.51[医药卫生—肿瘤]