烷化溶血磷脂对HL-60细胞生长及药物敏感性的影响  

EFFECTS OF ALP ON THE PROLIFERATION AND CHEMOSENSITIVITY OF HL 60 CELLS

作　　者：黄慧芳[1] 陈志哲[1] 卓光生[1] 王少元[1] 

机构地区：[1]福建医科大学附属协和医院,福建福州350001

出　　处：《白血病．淋巴瘤》2001年第1期19-21,共3页Journal of Leukemia & Lymphoma

摘　　要：目的 :探讨烷化溶血磷脂 ( AL P)对 HL 6 0细胞生长及药物敏感性的影响。方法 :以 AL P对 HL 6 0细胞克隆形成率及生长曲线影响来反映它的抗增殖效应。用 MTT法检测 HL 6 0细胞对 AL P及其他化疗药物敏感性。结果 :AL P能显著抑制 HL 6 0细胞的增殖能力 ,并且呈时间、剂量依赖性。 AL P与阿糖胞苷、氨甲蝶呤联合用药产生相加效应 ;与柔红霉素、地塞米松、三尖杉酯碱产生协同效应。结论 :AL P可抑制 HL - 6 0细胞增殖 ,增强 HL - 6Objective:To explore the effects of ALP on the proliferation and chemosensitivity of HL 60 cells. Methods:Proliferative potential was measured by colony formation assays and growth curves. Chemosensitivity was detected by the MTT colorimetric assays.Results:The result obtained by liquid suspension culture and semi solid colony culture indicated that ALP exerted the inhibitory effects also in a dose dependent and time dependent manner on HL 60 cells proliferation.15μg/ml ALP treatment of HL60 cells over a period of 24h could eliminate 100% of clonogenic HL60 cells.Enhanced antiproliferative activity was found in combinations of ALP with other antineoplastic drugs.An additive interaction exists in combination of ALP with Ara C、MTX;a synergism was found in combination of ALP with DNR、DEX、H.Conclusion:The results of this study strongly support that ALP could be an excellent antileukemic drug.The use of ALP may be a promising approach for marrow pruging.Further clinical trials of these compounds should be pursued.

关 键 词：抗肿瘤药物 醚酯 烷化溶血磷酯 HL-60细胞生长 白血病 

分 类 号：R730.5[医药卫生—肿瘤]