透明质酸-葡聚糖超分子水凝胶的制备及其细胞包载性能研究  

Preparation of HA-Dex supramolecular hydrogel for cell encapsulation

作　　者：曹璐娟 施瑜 陈荆晓 陈敬华 

机构地区：[1]江南大学药学院,无锡214122

出　　处：《中国药科大学学报》2014年第3期307-313,共7页Journal of China Pharmaceutical University

基　　金：教育部博士点基金资助项目(No.20110093110008);江苏省自然科学基金资助项目(No.BK2012557);武汉大学生物医用高分子材料教育部重点实验室开放基金资助项目(No.20110401)~~

摘　　要：分别制备了经β-环糊精(β-CD)修饰的透明质酸主体大分子(HA-CD),用2-萘乙酸(2-NAA)修饰的葡聚糖客体大分子(Dex-NAA),其结构均经核磁共振氢谱进行确认,接枝率分别为15.53%和7.38%。利用β-环糊精和2-萘乙酸间的主客体识别作用,制备得到超分子水凝胶。扫描电镜观测表明水凝胶内部为多孔结构,并且排列规整;水凝胶经流变学测试其储存模量大于损耗模量,具有较好的机械强度;同时,水凝胶具有良好的细胞相容性,可有效包裹细胞并促进细胞增殖生长。这种由天然多糖构建的超分子水凝胶有望作为细胞支架应用于组织工程领域。In this study, hyaluronic acid/β-cyclodextrin conjugate（HA-CD） and dextrin/2-naphthylacetic acid conjugate（Dex-NAA） were prepared, respectively. Their chemical structures were confirmed by ^1 H NMR, the degrees of substitution（DS） of HA-CD and Dex-NAA were determined to be 15.53% and 7.38%, respectively. A supramolecular hydrogel was subsequently prepared by the host-guest interaction between β-CD and 2-NAA based on these two gelators. Based on scanning electron microscopy（SEM） observation illustrated that the hydrogel had regularly porous architecture. Rheological analysis exhibited that the storage modulus of the hydrogel was higher than the loss modulus, indicating good mechanical strength. Meanwhile, the hydrogel showed favorable biocompatibility and could, therefore, encapsulate cells in situ, leading to promotion of cell proliferation and growth. This natural polysaccharide-based supramolecular hydrogel is promising in the application of tissue engineering as cell scaffold.

关 键 词：水凝胶 细胞包载 透明质酸 Β-环糊精 主客体作用 

分 类 号：R943[医药卫生—药剂学]