RANTES、MIP-1α、MIP-1β和MCP-1趋化因子在慢性肝病患者的水平  

The expression levels of chemokine RANTES, MIP-1α, MIP-1β and MCP-1 in chronic hepatitic diseases patients

作　　者：刘宁[1] 江娜[1] 罗娜[1] 乔桂芳[1] 孙焕芹[1] 刘金花[1] 张永宏[1] 

机构地区：[1]首都医科大学附属北京佑安医院生物医学信息中心,100069

出　　处：《北京医学》2014年第6期426-428,共3页Beijing Medical Journal

基　　金：北京市科技计划课题(D131100005313004;D131100005313005);国家国际科技合作专项项目(2012DFA30850)

摘　　要：目的探讨慢性乙型肝炎(CHB)、乙肝肝硬化(LC)、乙肝相关肝细胞癌(HCC)患者体内正常T细胞表达和分泌调节活化因子(RANTES)、巨噬细胞炎症蛋白(MIP-1α和MIP-1β)和单核细胞趋化蛋白(MCP-1)的表达水平。方法收集2010年2月至2011年11月首都医科大学附属北京佑安医院收治的CHB患者(40例)、LC患者(40例)、HCC患者(53例)的血液标本,采用Luminex技术检测血清趋化因子RANTES、MIP-1α、MIP-1β和MCP-1的表达水平,并以25例健康志愿者血液标本作为正常对照。结果趋化因子MIP-1α、MIP-1β和MCP-1在正常对照组表达水平的中位数分别为9.590、106.490和72.330 pg/ml,高于CHB组、LC组和HCC组;趋化因子RANTES在HCC组的表达水平为16 948.440 pg/ml,依次高于正常对照组、CHB组、LC组,差异有统计学意义(α<0.008)。结论趋化因子RANTES的表达水平与乙肝相关HCC的发生关系密切,可以作为HCC发生发展的辅助监测指标。Objective To study the expression levels of regulated upon activation normal T cell expressed and se-creted factors （RANTES）, macrophages inflammatory protein （MIP-1α and MIP-1β） and monocyte chemoattractant protein （MCP-1） by in chronic hepatitis B （CHB）, HBV-related liver cirrhosis （LC）, HBV-related hepatocellular carcinoma （HCC） patients. Methods Blood samples of 40 cases of CHB, 40 cases of LC, 53 cases of HCC from February 2010 to Novem-ber 2011 in Beijing You＆#39;an hospital were collected. The expression levels of RANTES, MIP-1α, MIP-1β and MCP-1 in patients serum were detected by Lumine＆#39;s technique. Blood samples of 25 healthy volunteers served as normal controls. Results Chemokines MIP-1α, MIP-1β and MCP-1 expression levels in the normal control group was 9.590, 106.490, 72.330 pg/ml, which was higher than LC, HCC and CHB group respectively. Chemokine RANTES expres-sion level in the HCC group was 16 948.440 pg/ml, which was higher than normal control group＆gt;CHB group＆gt;LC group. There was significant difference （α＆lt; 0.008） respectively. Conclusion The expression level of chemokines RANTES and the occurrence of HBV-related HCC is closely related. RANTES can be used as indicator in monitoring the occurrence of HBV-related HCC.

关 键 词：慢性乙型肝炎 肝硬化 肝细胞癌 正常T细胞表达和分泌调节活化因子 巨噬细胞炎症蛋白 单核细胞趋化蛋白 

分 类 号：R575[医药卫生—消化系统]