原发性胆汁性肝硬化患者外周血Th22细胞水平的变化及意义  

Change of Th22 cells in peripheral blood of patients with primary biliary cirrhosis and clinical implication

作　　者：彭桉平[1] 陈曲波[1] 侯敬侠 卢欣沂 赵蓉[1] 赵莉[3] 

机构地区：[1]广州中医药大学第二临床医学院检验科,510120 [2]山东省枣庄市立医院检验科,277100 [3]广州医科大学药学院蛇毒研究所,510182

出　　处：《实用医学杂志》2014年第12期1987-1989,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金青年基金(编号:81303287);广东省医学科研基金(编号:A2013236)

摘　　要：目的:观察Th22细胞在原发性胆汁性肝硬化(PBC)患者外周血中的变化,探讨其对发病的作用。方法:流式细胞术检测PBC患者组与健康体检组(对照组)外周血中Th22细胞比例;ELISA检测血浆IL-22水平,全自动生化仪检测ALT、AST、GGT、TBIL和CRP等临床实验室指标,并进行相关分析。结果:PBC患者组Th22细胞比例明显高于对照组(P<0.05),且肝硬化的PBC患者组高于非肝硬化的PBC患者组(P<0.05);血浆IL-22表达水平,临床指标ALT、AST、GGT、TBIL和CRP,PBC患者组明显高于对照组(P<0.05),且Th22细胞比例与AST,ALT、GGT和CRP呈正相关(P<0.05)。结论:PBC患者Th22细胞可能参与PBC的发病机制,有可能成为一个潜在的治疗靶点。Objective To investigate the change of Th22 cells in the peripheral blood of the patients with primary biliary cirrhosis （PBC） and evaluate its clinical significance. Methods The proportion of Th22 cells in the peripheral blood of PBC patients and healthy controls were evaluated by flow cytometry. The cytokine IL-22 of each group was measured by ELISA and ALT, AST, GGT, TBIL and CRP were measured by Automatic biochemical analyzer. The proportion of Th22 cells correlation with IL-22, ALT, AST, GGT, TBIL and CRP were analyzed. Results The proportion of Th22 cells was higher in PBC patients than healthy controls （P 〈 0.05）, Moreover the frequency of Th22 was increased in PBC patients with liver cirrhosis than in PBC patients with liver non-cirrhosis （P 〈 0.05）. The level of IL-22, ALT, AST, GGT, TBIL and CRP were increased in PBC patients （P 〈 0.05）. Moreover Th22 frequency of peripheral blood was positively associated with IL-22, ALT, AST, GGT and CRP （P 〈 0.05）. Conclusion Th22 may be involved in the pathogenesis of and it is a potential therapy target for PBC.

关 键 词：原发性胆汁性肝硬化 辅助性T细胞22 

分 类 号：R575.2[医药卫生—消化系统]