新疆维吾尔族宫颈癌组织中HPV-DNA表达及存在状态的研究  

Expression and physical status of HPV16-DNA in cervical cancer tissue of Xinjiang Uyghur patients

作　　者：高冬梅[1] 龙梅[2] 张璐[1] 张媛媛[1] 古扎丽努尔.阿不力孜 

机构地区：[1]新疆医科大学附属肿瘤医院妇科,乌鲁木齐830011 [2]新疆医科大学第一附属医院妇科.生殖中心,乌鲁木齐830054

出　　处：《新疆医科大学学报》2014年第7期837-840,共4页Journal of Xinjiang Medical University

基　　金：新疆科技厅地方病分子生物学实验室开放课题(XJDX0208-2013-03)

摘　　要：目的探讨高危型人乳头瘤病毒(HR-HPV)在新疆维吾尔族宫颈癌患者中的表达、存在状态及其与宫颈癌的相关性。方法选择第2代杂交捕获(Hybrid capture,HC2)检测HR-HPV阳性的30例维吾尔族宫颈鳞癌(SCC)患者,利用巢式PCR检测其组织标本中的HPV16-DNA的表达,多重Taqman探针荧光定量PCR检测HPV16-DNA在组织中的存在状态。结果巢式PCR检测结果显示宫颈鳞癌患者HPV16-DNA检出率为96.7%,与HC2法的HPV-DNA检出率差异无统计学意义(P>0.05);HPV16-DNA在宫颈癌组织中以整合态存在,其整合率为89.7%;HPV16-DNA的表达水平与宫颈癌患者的年龄、有无淋巴结转移无关(P>0.05),但其在病理分级高的患者中表达高于病理分级低的患者(P<0.05),在临床分期高的患者中表达高于低分期患者(P<0.05)。结论 HR-HPV感染是导致宫颈癌的主要因素,而HPV16是新疆维吾尔族宫颈癌主要的病毒类型,其在组织中多以整合态存在,在宫颈癌发病中起主要作用,HPV-DNA整合状态与宫颈细胞的恶性转化密切相关。Objective To explore the expression and infection state of HR-HPV in cervical cancer tissues and its relationships between cervical cancer occurrence and HPV infection. Methods HR-HPV infection was detected by HC2; 30 cases of HR-HPV positive cervical cancer tissues were collected. The expression of HPV16-DNA was detected by the nested PCR and existing state in the organization of HPV16-DNA was detected by the multiple fluorescent quantitative PCR Taqman probe. Results HPV-16 positive rate in patients with cervical squamous cell carcinomas （SCC） was 96.7%, compared with HC2 detection rate, with no significant correlation （P 〉0.05） ; the HPV16-DNA existed in integrate state in cervical cancer tissues, with the integrate rate of 89.7%. Conclusion HR-HPV infection was the main factor leading to cervical cancer, and HPV-16 was the main type of viruses in Xinjiang Uygur cervical cancer. Its integration status in the organization played a main role in the incidence of cervical cancer and was closely related to the malignant transformation of cervical cells.

关 键 词：宫颈癌 HPV—DNA 表达 整合 

分 类 号：R737.33[医药卫生—肿瘤] R365[医药卫生—临床医学]