BMP-2、bFGF和Dex联合应用对犬牙周膜细胞增殖及矿化结节形成能力的影响  

Osteogenic differentiation of dog periodontal ligament cells induced by BMP-2、bFGF and Dex

作　　者：宋扬 秦青 高菊荣 王丽颖[2] 刘佳[2] 金作林 

机构地区：[1]解放军323医院口腔科,主治医师陕西710054 [2]第四军医大学口腔医学院正畸科,博士陕西710032 [3]第四军医大学口腔医院正畸科,副主任医师教授陕西710032

出　　处：《中华老年口腔医学杂志》2014年第2期80-84,共5页Chinese Journal of Geriatric Dentistry

基　　金：国家自然科学基金资助项目(项目编号:30973358);陕西省自然科学基础研究计划(项目编号:2014JM4111)

摘　　要：目的:联合应用BMP-2、bFGF和Dex诱导牙周膜细胞(PDLCs),观察其成骨分化能力。方法:体外培养犬PDLCs,将第4代PDLCs进行分组:(1)对照组增加矿化诱导液组(2)bFGF+Dex组、(3)BMP-2+bFGF组、(4)加BMP-2+Dex组、(5)bFGF+BMP-2+Dex组。MTT法观察BMP-2、bFGF和Dex联合应用对犬PDLCs增殖的影响;成骨分化实验观察BMP-2、bFGF、Dex联合应用对犬PDLCs分化的影响。结果:(1)PDLCs细胞呈典型的成纤维细胞样形态:长梭形、纺锤形、三角形等多形性。波形丝蛋白表达阳性,角蛋白表达阴性。(2)MTT增殖实验显示:bFGF+Dex、BMP-2+bFGF、BMP-2+Dex、bFGF+BMP-2+Dex组均促进犬PDLCs增殖,但各诱导组间无明显差异。(3)成骨诱导实验检测结果:bFGF+BMP-2+Dex组矿化结节染色深、面积较大、细胞密集而集中。结论:三种诱导因子两两结合均能增强犬PDLCs矿化结节形成能力,但三种因子联合应用成骨能力最强(P<0.05)。Objective： Osteogenic differentiation of periodontal ligament cells induced by BMP-2, bFGF and Dex in dogs. Methods： The fourth generation of PDLCs cultured in vitro were randomly divided into 5 groups： （1） the control group （with no inducible factors）; （2） the group with 10p g/L bFGF and 200p g/L BMP-2 added; （3） the group with 200p g/L BMP-2 and 10-Smol/L Dex added; （4） the group with 10p g/L bFGF and 10-8mol/L Dex added; （5） the group with 200p g/LBMP-2, 10p g/L bFGF and 10-Smol/L Dex added. The effect of combined use ofBMP-2, bFGF and Dex on the proliferation of PDLCs in dogs were observed by MTT test, and osteogenic differentiation test assay. Results： （1） After 7, 14 days, the induced PDLCs differentiated into fibroblast-like cells with polygon in shape including triangle and fusiform. Vimentin expressed positive and keratin was negative; （2）MTT test demonstrated that all of the four experimental groups promoted the proliferation of PDLCs in dogs and the effect were time-dependent; （3） Osteogenesis induced by 31 d, bFGF＋BMP-2＋Dex mineralized nodule staining deep, larger cell density and concentrated. Conclusion： These results indicate that combined application BMP-2, bFGF and Dex can remarkably promote the osteogenic differentiation of PDLCs.

关 键 词：牙周膜细胞 骨形成蛋白-2 碱性成纤维细胞生长因子 地塞米松 

分 类 号：R781.4[医药卫生—口腔医学]