TRAIL联合多西紫杉醇诱导喉鳞癌Hep-2细胞凋亡的实验研究  

Effects of TRAIL and Docetaxel on Cellular Apoptosis of Human Laryngeal Squamous Carcinoma Cells

作　　者：姚鸿超[1] 李秋影[1] 李茗华[1] 冯佳鹏[1] 王婧婷[1] 肖辉[1] 

机构地区：[1]哈尔滨医科大学附属第二医院耳鼻咽喉-头颈外科,黑龙江哈尔滨150086

出　　处：《现代生物医学进展》2014年第19期3619-3621,3630,共4页Progress in Modern Biomedicine

基　　金：黑龙江省教育厅科学技术研究项目(11541177)

摘　　要：目的:观察肿瘤坏死因子相关凋亡诱导配体(TRAIL)联合多西紫杉醇应用于人喉鳞癌Hep-2细胞生长的抑制增殖和诱导凋亡作用。方法:实验分四组,1组对照组,2组为应用TRAIL组,3组单独应用多西紫杉醇,4组联合应用TRAIL及多西紫杉醇。分别应用MTT、流式细胞仪检测细胞凋亡率,倒置显微镜观察细胞的形态学改变。结果:TRAIL与多西紫杉醇联合作用于Hep-2细胞,能显著增强对Hep-2细胞的杀伤、抑制增殖及诱导凋亡作用,其联合应用的凋亡抑制率明显高于单独应用TRAIL组和多西紫杉醇组(P<0.05)。结论:TRAIL与多西紫杉醇联用能显著提高对喉鳞癌Hep-2细胞的生长抑制和诱导凋亡作用。Objeelive： To investigate the apoptosis inducing effect by tumor necrosis factor related apoptosis-ligand（TRAIL）and docetaxel on human laryngeal squarnous carcinoma Hep-2 cells. Methods： Four groups were divided to conduct the experiment： Group 1 as control group, TRAIL applied to Group 2, docetaxel applied to Group 3, and TRAIL combined with docetaxel for Group 4.Apoptosis rate was detected by using MTT and FCM, and mophological changes were observed with inverted microscope. Results： TRAIL and docetaxel combination in Hep 2 cells, can significantly enhance the Hep-2 cells of destruction, inhibit proliferation and induce apoptosis, apoptosis inhibition rate of the joint application was obviously higher than that of single application of TRAIL and docetaxel group （P〈0. 05）. Conclusion＂ The combination of TRAIL and doeetaxel could significantly increase apoptosis effects on human laryngeal squamous carcinoma cells.

关 键 词：肿瘤坏死因子相关诱导凋亡配体 多西紫杉醇 HEP-2细胞 凋亡 

分 类 号：R739.65[医药卫生—肿瘤]