PPARγ、PTEN、Akt在胃癌中的表达及其与胃癌生物学行为的关系  

Relationship between expression of PPARγ,PTEN and Akt and biologic behaviors of gastric carcinoma

作　　者：廖山婴[1] 朱小波[1] 王蓓蓓[1] 马娟[1] 沙卫红[1] 

机构地区：[1]广东省人民医院(广东省医学科学院)消化内科,广州510080

出　　处：《新医学》2014年第6期394-398,共5页Journal of New Medicine

基　　金：广东省医学科研基金(B2011010)

摘　　要：目的检测人胃癌组织中过氧化物酶增殖激活受体γ(PPARγ)、10号染色体缺失张力蛋白同源的磷酸酶基因(PTEN)、丝氨酸/苏氨酸蛋白激酶(Akt)mRNA表达,探讨其与胃癌生物学行为的关系。方法应用逆转录PCR法检测60例人胃癌组织及相应癌旁组织,20名正常胃组织中PPARγ、PTEN、Akt mRNA的表达情况,并分析三者与胃癌分化程度、肿瘤大小、淋巴结转移、远处转移、浸润深度和临床分期及患者性别、年龄之间的关系,同时分析三者的相关性。结果胃癌组织中PPARγ及Akt mRNA表达水平高于癌旁组织及正常胃组织(P<0.05),而PTEN mRNA表达水平明显较癌旁组织及正常胃组织低(P<0.05),在癌旁组织与正常胃组织间PPARγ、PTEN、Akt mRNA的表达水平均无明显差异。胃癌组织中PPARγ、PTEN、Akt mRNA表达量与患者性别、年龄、肿瘤大小及分化程度无关,而与胃癌的TNM分期、侵犯浆膜、淋巴结转移及远处转移相关(P<0.05)。PPARγ与PTEN的表达呈负相关(rs=-0.492,P<0.05),与Akt表达呈正相关(rs=0.623,P<0.05),PTEN与Akt表达呈负相关(rs=-0.565,P<0.05)。结论 PPARγ、Akt在胃癌中高表达而PTEN呈低表达,三者中存在明显相关,联合检测可作为判断胃癌恶性程度和预后的指标。Objective To investigate the relationship between expression of peroxisome proliferator-activated receptors γ（PPARγ）,phosphatase and tensin homology deleted on chromosome 10 （PTEN）and Akt and biological behaviors of gastric carcinoma. Methods The expression of PPARγ,PTEN and Akt mRNA in 60 cases of gastric carcinoma and the corresponding non-carcinoma tissues and 20 cases of normal gastric tissues were detected by reverse transcription polymerase chain reaction （RT-PCR ）. The correlation of PPARγ,PTEN and Akt expression with tumor differentiation grade,size,lymph node metastasis,distant metastasis,depth of invasion,TNM stage and patients’gender and age were analyzed. Results PPARγand Akt expression in gastric carcinoma were significantly higher but PTEN expression significantly lower than in corre-sponding non-carcinoma tissues and normal gastric tissues （P〈0.05 ）. There was no significant difference in the expression of PPARγ,PTEN and Akt mRNA in non-carcinoma tissues and normal gastric tissues. PPARγ, PTEN and Akt expression in gastric carcinoma were significantly associated with TNM stage,serosal invasion, lymph node metastasis and distant metastasis （P〈0.05 ）,but not correlated with the gender,age,tumor size and differentiation grade. The expression of PPARγwas negatively correlated with PTEN （rs =-0.492,P〈0.05）but positively with Akt （rs =0.623,P〈0.05）. Moreover,the expression of PTEN was negatively cor-related with Akt （rs =-0.565,P〈0.05）. Conclusion PPARγand Akt are highly expressed in gastric car-cinoma,while PTEN is lowly expressed in gastric carcinoma and there is a significant correlation between them.They may serve as markers in the diagnosis of gastric cancer and estimation of prognosis.

关 键 词：PPARΓ PTEN AKT 胃癌 临床病理参数 

分 类 号：R735.2[医药卫生—肿瘤]