乳腺癌组织中CD68、B细胞淋巴瘤基因2及VEGF165的表达及其临床意义  

Expressions of CD68,Bcl-2 and VEGF165 in breast cancer and their clinical significance

作　　者：安杰[1] 刘伟[2] 刘爽[1] 

机构地区：[1]中国人民解放军白求恩国际和平医院病理科,石家庄050082 [2]中国人民解放军白求恩国际和平医院普外一科,石家庄050082

出　　处：《中华乳腺病杂志（电子版）》2014年第2期29-32,共4页Chinese Journal of Breast Disease(Electronic Edition)

摘　　要：目的研究抗原CD68、B细胞淋巴瘤/白血病-2基因(B cell lymphoma/leukemia-2,Bcl-2)及VEGF165在乳腺癌组织中的表达及其临床意义。方法收集2012年3月至2013年3月本院40例手术切除浸润性乳腺癌标本,应用免疫组织化学法检测40例乳腺癌和癌旁组织中CD68、Bcl-2及VEGF165的表达。计量资料采用单因素方差分析,计数资料采用χ2检验或Fisher确切概率法。相关性分析采用Pearson相关分析法。结果乳腺癌组织中CD68、Bcl-2及VEGF165阳性表达率分别为70.0%(28/40)、77.5%(31/40)、80.0%(32/40),癌旁组织中分别为10%(4/40)、57.5%(23/40)、15.0%(6/40),差异有统计学意义(t=20.637,21.877,35.104,P均=0.000)。CD68、Bcl-2、VEGF165在浸润性乳腺癌组织中的表达与患者的年龄、有无脉管癌栓、ER、PR表达无关,而与乳腺癌的TNM分期(r=4.571、4.167、4.167,P=0.033、0.041、0.041)、淋巴结转移(r=8.127,P=0.004;χ2=5.974,P=0.015;P=0.036))有关(P<0.05)。Pearson线性相关分析显示乳腺癌组织中CD68与Bcl-2之间(r=0.793,P=0.000),CD68与VEGF165之间(r=0.494,P=0.002),Bcl-2与VEGF165之间(r=0.507,P=0.001)均呈正相关。结论乳腺癌组织CD68、Bcl-2及VEGF165过表达,三者可作为判断乳腺癌病情的参考指标。Objective To explore the expressions of CD68, B cell lymphoma/leukemia-2（Bcl-2） and VEGF165 in breast cancer tissue and their clinical significance. Methods We collected the surgically resected specimens from 40 cases of invasive breast cancer from March 2012 to March 2013 in our hospital. The expressions of CD68, Bcl-2 and VEGF165 were detected by immunohistochemistry in breast cancer tissue and adjacent normal tissue of the 40 cases. Measurement data was processed using one-way ANOVA, numeration data using the chi-square test or Fisher ’ s exact method. Correlation analysis was performed using Pearson correlation analysis. Results The positive expression rates of CD68, Bcl-2 and VEGF165 were 70. 0% （28/40）, 77. 5% （31/40）, 80. 0% （32/40） in breast cancer tissue, and 10%（4/40）, 57. 5% （23/40）, 15. 0% （6/40） in adjacent tissues respectively, suggesting that the difference was statistically significant （ t=20. 637, 21. 877, 35. 104, all P=0. 000） . The expressions of CD68, Bcl-2 and VEGF165 in invasive breast cancer tissue were not correlated with the patient ’ s age, vascular invasion, ER and PR expression, but correlated with TNM stage （r=4. 571,4. 167,4. 167, P=0. 033, 0. 041, 0. 041） and lymph node metastasis （r=8. 127, P=0. 004;χ^2=5. 974, P=0. 015;P=0. 036）. Pearson linear correlation analysis showed that in breast cancer tissue, there was a positive correlation between Bcl-2 and CD68 （r=0. 793, P=0. 000）, between CD68 and VEGF165 （r=0. 494, P=0. 002）, and between Bcl-2 and VEGF165 （r=0. 507, P=0. 001）. Conclusion CD68, Bcl-2 and VEGF165 are over-expressed in breast cancer tissue, which can provide references to the evaluation of breast cancer.

关 键 词：乳腺肿瘤 血管内皮生长因子 巨噬细胞 基因  BCL-2 CD68 

分 类 号：R737.9[医药卫生—肿瘤]