某屠宰场猪粪便中单增李斯特菌携带调查  

Investigation of Listeria monocytogenes in Swine Stool in an Abattoir

作　　者：刘凯[1] 王艳[1] 王天姝 贺春月[3] 代航[1] 于波[1] 袁雪娇[1] 叶正兴[1] 王毅[1] 许华青 孟双[1] 叶长芸[1] 

机构地区：[1]传染病预防控制国家重点实验室中国疾病预防控制中心传染病预防控制所,北京102206 [2]贵州省黔南州人民医院检验科,贵州558000 [3]湖南省怀化市妇幼保健院,湖南怀化418000 [4]贵阳医学院微生物学教研室,贵州贵阳550004

出　　处：《中国动物检疫》2014年第6期47-49,67,共4页China Animal Health Inspection

基　　金：国家重大传染病防治科技重大专项(2011ZX10004-001;2013ZX10004-101)资助

摘　　要：为了初步探索猪的李斯特菌携带情况以及屠宰加工环境中污染的可能性,并进一步对分离到的单增李斯特菌进行分型分析,以了解其分子流行病学特征,为预防控制食源性李斯特菌病提供参考。2011年3月至2012年2月每月月初定点采集某一屠宰场的猪粪便标本和加工场所环境水样标本,进行李斯特菌的病原学分离,对分离到的单增李斯特菌进行血清分型、多位点序列分型(MLST)和脉冲场电泳分型(PFGE)分析。1322份猪粪便标本和104份环境标本中,共分离到7株单增李斯特菌、56株无害李斯特菌。5株单增李斯特菌属于1/2c血清型(ST9型),1株1/2a血清型(ST199型),1株1/2b血清型(ST5型)。4株1/2c型菌株具有完全相同的PFEG带型,与另外1株1/2c型菌株具有相似带型,而与其他2株菌株(分别为1/2a型和1/2b型)的带型具有较大差异。猪的单增李斯特菌携带率较低,但存在污染加工环境的可能性,进而导致生猪肉食品受到污染。对屠宰及加工场所采取有效的消毒灭菌措施能够避免单增李斯特菌的污染和传播。To find out the Listeria infection in swine and the contamination risks in the processing environment,1322 stools of swine and 104 environmental samples were collected for the isolation of Listeria from March 2011 to February 2012. Seven Listeria monocytogenes and fifty-six Listeria innocua were identified. In order to understand the epidemiological characteristic of L. monocytogenes,all the seven isolates were subtyped by serotyping,multi-locus sequence typing（MLST）and pulse field gel electrophoresis（PFGE）. Five L. monocytogenes strains belonged to 1/2c（ST9）,the rest two strains were 1/2a（ST199）and 1/2b（ST5）,respectively. Four of five 1/2c serotype strains showed the same pulsotype,which was very similar with the remaining 1/2c serotype strain. The other two strains（1/2a and 1/2b serotype）,showed distinct pulsotypes from that of 1/2c strains. The result showed that the infection rate of L. monocytogenes in swine was low, and the contamination of environment could possibly happen if the infected swine enter the process. The contamination and spreading of L. monocytogenes could be avoided by proper disinfection in slaughterhouse.

关 键 词：单增李斯特菌 猪粪便标本 分子分型 

分 类 号：R378.994[医药卫生—病原生物学]