新疆细虫草抑菌及抗氧化作用的初步研究  

Antibacterial and Antioxidant Activities of a Ophiocordyceps gracilis Strain from Xinjiang

作　　者：索菲娅[1] 黄罗冬[2] 李萍[1] 张琳[1] 王傲立[1] 

机构地区：[1]新疆大学生命科学与技术学院,新疆乌鲁木齐830046 [2]云南大学中草药生物资源研究所云百草实验室,云南昆明650091

出　　处：《食用菌学报》2014年第1期51-54,共4页Acta Edulis Fungi

基　　金：新疆维吾尔科技支疆项目(编号:201091245);国家自然科学基金(编号:31160073);新疆教育厅高等学校科研计划(编号:XJEDU2010I12)的部分研究内容

摘　　要：分别采用滤纸片琼脂扩散法和二苯代苦味基肼自由基(DPPH)法,对新疆细虫草(Ophiocordyceps gracilis)水提物和醇提物的体外抑菌和抗氧化活性进行研究。结果表明,新疆细虫草水提物和醇提物对大肠杆菌、金黄色葡萄球菌、枯草芽孢杆菌、变形杆菌均有很好的抑制效果,醇提物的抑菌效果明显高于水提物;新疆细虫草水提物和醇提物均有很好的DPPH自由基清除能力,IC50值分别为1.77 mg/mL和1.23mg/mL,清除能力随浓度增大而增强,当提取液浓度为5mg/mL时新疆细虫草水提物和醇提物的清除率均达到了97%。Antibacterial and antioxidant activities of ethanolic and aqueous extracts of Ophiocordyceps gracilis fruit bodies collected from the wild in Xinjiang Province were investigated using the paper disc diffusion and the 2,2-diphenylpicrylhydrazyl （DPPH）radical scavenging methods.Both aqueous and especially ethanolic extracts exhibited significant antibacterial activity towards Escherichia coli,Staphyloccocus aureus,Bacillus subtilis and Proteus vulgaris.Both extracts also exhibited dose-dependent DPPH radical scavenging activity with IC50 values of 1 .77 mg/mL and 1 .23 mg/mL recorded for the aqueous and ethanolic extracts respectively,and each extract at 5 mg/mL concentration resulted in a 97% radical scavenging rate.

关 键 词：细虫草 醇提物 水提物 抑菌 抗氧化 新疆 

分 类 号：S567.35[农业科学—中草药栽培]