LCM技术筛选胃癌相关蛋白质  

Screening of Gastric Carcinoma Related Proteins by LCM

作　　者：李素云[1] 陈苏琼[1] 谢远杰[1] 张志伟[1] 贺修胜[1] 

机构地区：[1]南华大学肿瘤研究所,湖南衡阳421001

出　　处：《中南医学科学杂志》2014年第3期233-236,共4页Medical Science Journal of Central South China

基　　金：湖南省自然科学基金资助项目(12JJ3102);湖南省卫生科研基金资助项目(B2012-046);湖南省高校创新平台开放基金资助项目(13K082);地方高校国家级大学生创新创业训练计划资助项目(201210555017)

摘　　要：目的比较分析胃癌与正常胃黏膜组织间差异,筛选与纯化出胃癌相关蛋白质。方法应用冰冻切片、苏木素-伊红染色、甲基绿染色、激光捕获显微切割(LCM)技术,获取高纯度的胃癌组织与正常胃黏膜的腺体组织,提取LCM纯化后组织细胞总蛋白,测定总蛋白浓度。结果快速制作出正常胃黏膜上皮和胃腺癌组织快速冰冻切片和HE病理切片,高纯度纯化胃腺癌细胞(GAC)和正常胃黏膜上皮细胞(NGEC),得出蛋白定量标准曲线与直线回归方程。结论运用LCM可高纯度筛选胃腺癌差异表达蛋白质,此结果为寻找胃癌早期诊断及治疗的蛋白质分子标志物提供前期基础。Objective To compare and analyze the differences between normal gastric mucosa tissue and gastric cancer,screen and purify gastric cancer related protein. Methods Through frozen section,Hematoxylin and eosin stai-ning,Methyl green staining and laser capture micro-dissection （ LCM ） , gastric cancer tissue and normal gastric mucosa gland tissue in high concentrations were obtained. LCM purified tissue cells＆#39;total protein was extracted,and its concentration was assayed. Results Normal gastric epithelium and gastric cancer tissue frozen sections and HE pathological sections were rapidly produced,gastric adenocarcinoma cells （ GAC） and normal gastric adenoepithelial cells （ NGEC） were purified in a high concentration. Standard curve and linear regression equation were obtained for quantitative determination of pro-tein. Conclusion Through LCM,differentially expressed protein of gastric cancer can be screened in high concentration, which would be a foundation for seeking protein molecular markers for early diagnosis and therapy of gastric cancer.

关 键 词：胃癌 蛋白质 激光捕获显微切割 筛选 纯化 

分 类 号：R735.2[医药卫生—肿瘤]