金樱子对大鼠糖尿病性白内障细胞凋亡和氧化应激的影响  

Effects of Rosa Laevigata on Diabetic Cataract in Rats Apoptosis and Oxidative Stress

作　　者：周俊[1] 何湘珍[1] 肖启国[1] 彭辉灿[1] 王俞方[1] 李洁[1] 

机构地区：[1]南华大学附属第二医院眼科,湖南衡阳421001

出　　处：《中南医学科学杂志》2014年第3期241-245,共5页Medical Science Journal of Central South China

基　　金：湖南省科技厅项目(编号:2014SK3021)

摘　　要：目的探讨金樱子对糖尿病大鼠晶状体上皮细胞凋亡的作用及氧化应激机制。方法用Streptozotocin(STZ)腹腔注射建立SD(Sprague-Dawley)大鼠糖尿病模型,然后将其随机分为糖尿病模型组及金樱子干预组,另设正常对照组。12周后,检测晶状体组织中总抗氧化能力(TAC)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量。采用流式细胞术检测大鼠晶状体上皮细胞凋亡率。结果金樱子能显著降低糖尿病大鼠晶状体上皮细胞凋亡率(P<0.05)。金樱子能显着降低糖尿病大鼠的血糖、血脂及MDA含量,并提高TAC和SOD活性(P<0.05)。结论金樱子能抑制晶状体上皮细胞凋亡和氧化应激,延缓糖尿病性白内障的发展。Objective To investigate the mechanism of the stress of Rosa laevigata onapoptosis in diabetic rats lens epithelial cells and the role ofoxidation. Methods SD （ Sprague-Dawley） model of diabetic rats was established using Streptozotocin （ STZ） intraperitoneal injection, and then randomly divided into intodiabetic model group and intervention group of Rosa laevigata. At the same time,the experimental control group was set. After 12 weeks,the rats were sacrificed, and their total antioxidant capacity assay lens tissues （TAC）,superoxide dismutase （SOD） activity and malondialdehyde （ MDA） content were measured. Lens epithelial cell apoptosis was detected by flow cytometry. Results Rosa laevigata could significantly decrease the apoptosis of lens epithelial cells of diabetic rat rate （P＆lt;0. 05）. Rosa laevigata significantly decreased the blood glucose,blood lipid and MDA in diabetic rats,and improved TAC and SOD activity （P＆lt;0. 05）.

关 键 词：金樱子 糖尿病性白内障 氧化应激 凋亡 

分 类 号：R776.1[医药卫生—眼科]