原发性肝癌组织中LASP-1 mRNA和HPA-1 mRNA表达水平及其临床意义  

Expression levels and clinical significance of LASP-1 mRNA and HPA-1 mRNA in primary hepatocellular carcinoma tissues

作　　者：王贺[1] 孙风波 邢智远 张凤娟[4] 

机构地区：[1]山东省青岛市传染病医院肝病六区,266033 [2]青岛市海慈医疗集团普外科 [3]青岛市中心医院普外科 [4]青岛大学医学院附属医院消化内科

出　　处：《中国临床研究》2014年第6期641-643,636,共3页Chinese Journal of Clinical Research

摘　　要：目的定量检测LIM和SH3蛋白1(LASP-1)mRNA和乙酰肝素酶-1(HPA-1)mRNA在原发性肝癌组织中的表达水平,探讨二者与肝癌发生、侵袭和转移的关系。方法利用实时荧光定量PCR检测法分别检测62例原发性肝癌癌组织、配对癌旁组织(距癌2 cm)和正常肝脏组织(距癌大于5 cm)中LASP-1mRNA和HPA-1 mRNA的表达水平,并分析二者与肝癌临床病理特征的关系。结果肝癌组织、癌旁组织、正常肝组织中HPA-1 mRNA表达水平分别为47.25±16.65、23.15±14.89、7.56±5.15,LASP-1 mRNA的表达水平分别为51.66±20.12、35.73±15.07、10.17±8.29。HPA-1 mRNA及LASP-1 mRNA在癌组织中的表达高于癌旁组织和正常组织(P均<0.05)。HPA-1及LASP-1的表达与肿瘤分化程度、浸润深度、淋巴结转移及TNM分期密切相关(P<0.05,P<0.01),与患者年龄、性别及肿瘤大小无关(P均>0.05)。Spearman等级相关分析显示LASP-1 mRNA和HPA-1 mRNA的表达呈正相关(OR=0.421,P<0.05)。结论肝癌组织中LASP-1 mRNA与HPA-1 mRNA的表达与肿瘤浸润深度、分化程度、淋巴结转移及TNM分期等密切相关。二者联合表达水平的增加可能促进肝癌的侵袭转移。Objective To detect quantitatively the expression levels of LIM and SH3 protein-1 （ LASP-1 ） mRNA and heparanase-1 （HPA-1） mRNA in primary hepatocellular carcinoma tissues and investigate the relationship between their expressions and genesis, invasion and metastasis of hepatocellular carcinoma. Methods SYBR Green I real-time fluorescent quantitative reverse transcriptase polymerase chain reaction （RT-PCR） was used to detect the expression levels of LASP-1 mRNA and HPA-1 mRNA in hepatocellular carcinoma tissues, paired adjacent hepatocellular carcinoma tissues（2 cm from carcinoma） and normal hepatic tissues （more than 5 cm from carcinoma） in 62 patients with primary hepatoma. The relationship between their expression levels and clinical pathological characteristics of hepatocellular carcinoma was analyzed. Results The expression levels （2 - △△ct ） of HPA-1 mRNA in hepatocellular carcinoma tissues, adjacent hepatocellular carcinoma tissues and normal hepatic tissues were 47.25±16.65,23.15 ± 14.89 and 7.56 ±5.15, respectively ; the expression levels （2 - △△ct ） of LASP- 1 mRNA in hepatocellular carcinoma tissues, adjacent hepatocellular carcinoma tissues and normal hepatic tissues were 51.66 ±20.12,35.73 ± 15.07 and 10.17± 8.29, respectively. The expression levels of HPA-1 mRNA and of LASP-1 mRNA in hepatocellular carcinoma tissues were all higher than those in adjacent hepatoeellular carcinoma tissues and normal hepatic tissues （ all P 〈 0.05 ）. The expression levels of LASP-1 mRNA and HPA-1 mRNA were closely associated with the degree of differentiation, depth of infiltration, lymph node metastasis and clinical staging of hepatic carcinoma （P 〈 0. 05 or P 〈 0.01 ）, but were not associated with age and sex of patient and size of hepatocellular carcinoma （ all P 〉 0.05 ）. The Spearman rank correlated analysis showed that the expressions of LASP-1 and HPA-1 were positive correlated （ OR = 0. 421, P 〈 0.05 ）. Conclusions The expression levels of LASP

关 键 词：原发性肝癌 LASP-1 HPA-1 实时荧光定量逆转录聚合酶链反应 侵袭 转移 

分 类 号：R735.7[医药卫生—肿瘤]