不同程度宫颈病变单一、多重高危型HPV感染状态下E6/E7 mRNA的表达  

Expression of E6/E7 mRNA in single or multiple high-risk HPV infection in different degrees of cervical lesions

作　　者：莫小亮[1] 罗殿中[2] 党裔武[2] 王莉[2] 

机构地区：[1]广西医科大学第一附属医院妇产科,南宁530021 [2]广西医科大学第一附属医院病理科,南宁530021

出　　处：《重庆医学》2014年第18期2268-2271,共4页Chongqing medicine

基　　金：广西自然科学基金资助项目(2013GXNSFAA019254)

摘　　要：目的探讨不同程度宫颈病变的HPV基因型分布特点及高危型HPV E6/E7mRNA在单一、多重感染病例中的表达,以及其对HPV致病力的影响。方法收集不同程度宫颈病变石蜡组织标本共236份。采用基因芯片技术对236份标本进行23种HPV基因型分型检测,并采用Taqman逆转录-PCR技术测定高危型HPV E6/E7mRNA的表达。结果 HPV阳性率为65.3%(154/236),其中包含了233个HPV亚型(单一或多重)感染,60.9%(142/233)病例可检测出E6/E7mRNA的表达,HPV阳性率和E6/E7mRNA表达水平随宫颈病变级别的上升而升高(P<0.05)。在大于或等于CINⅡ的高危亚型感染病例中,HPV45的E6/E7mRNA表达率最高(93.3%),其次为HPV16(81.0%)、HPV18(78.4%)。在大于或等于CINⅡ的病变中,93.7%(118/126)病例可检测到E6/E7mRNA的表达,而小于CINⅡ的病变中仅有39.3%(11/28)的病例可检测到E6/E7mRNA的表达。在所有不同程度宫颈病变中,多重感染病例E6/E7mRNA表达率较单一感染病例高(61.2%vs.38.8%,P<0.05)。结论在任何程度的宫颈病变中,HPV多重感染,尤其HPV45的E6/E7mRNA高表达水平可能会提高HPV的致病力。Objective To investigate the HPV genotype distribution characteristics in the patients with different degrees of cer-vical diseases and the expression of high-risk HPV E6/E7 mRNA in the patients with single or multiple infections and its influence on the HPV pathogenicity.Methods A total of 236 formalin-fixed,paraffin-embedded specimens were collected from women with different degrees of cervical diseases.The gene chip technique was adopted to conduct the genotype detection.The in 23 kinds of HPV genotype on 236 specimens.The TaqmanRT-PCR technique was adopted to measure the expression of high-risk HPV E6/E7 mRNA.Results The HPV positive rate was 65.3%（154/236）,including 233 cases of HPV subgenotye infection（single or multi-ple）,the expression of HPV E6/E7 mRNA was detected in 60.9% of cases（142/233）,the HPV positive rate and the expression level of HPV E6/E7 mRNA were increased with the increase of the cervical lesion grade（P＆lt;0.05）.In the high-risk infection cases of lesion≥CINⅡ,the expression rate of HPV45 E6/E7 mRNA was highest（93.3%）,followed by HPV16（81.0%）and HPV18 （78.4%）.In the lesions ≥CINⅡ,the expression of E6/E7 mRNA was detected in 93.7% of cases（118/126）,while which in le-sions ＆lt; CINⅡwas detected in only 39.3 % of cases（11/28）.In different degrees of cervical lesions,the expression rate of E6/E7 mRNA in the cases of multiple infection was higher than that in the caes of single infection（61.2%vs.38.8%,P＆lt;0.05）.Conclu-sion In any degree of cervical lesion,HPV multiple infection,especially high expression level of HPV45 E6/E7 mRNA could in-crease the HPV pathogenicity.

关 键 词：宫颈病变 HPV 基因型 E6/E7 MRNA 

分 类 号：R737.33[医药卫生—肿瘤]