人体基因组D14S13位点限制性片段长度多态性的研究  

The Study of the RFLP of D14S13 Site of Human Genome

作　　者：倪锦堂 姚翔 郭红玲 李伯龄 

机构地区：[1]公安部刑事技术研究所,北京100038

出　　处：《Acta Genetica Sinica》1993年第2期97-101,共5页

摘　　要：应用pCE1.2探针,分析了296个无关个体基因组D14S13位点HaeⅢ酶解的限制性片段长度多态性(RFLP),进行了等位基因频率的分布统计,发现至少含有66个等位基因,位点杂合性大于93%。在对8个家系93名相关个体的分析中,没有发现突变发生。此RFLP分析方法已初步应用于法医学的个人识别和亲权鉴定中,并对准确率进行了计算。The single-locus probe pCE 1.2 recognizes the DHS13 site in human genome. Allele-fre-quency of Haelll digested D14S13 site is drawn out from 269 unrelated individuals. It displayed that there were at least 66 alleles and more than 93% heterogeneity. No mutation in 93 individuals of 8 families was observed. This RFLP technique has been applied in the identification And parentage testing in forensic science, and the accuracy of identity was calculated. Some further applications were discused.

关 键 词：多态性 等位基因频率 RFLP 基因组 

分 类 号：Q987[生物学—遗传学]