超声波辅助双水相体系提取竹叶椒总生物碱的工艺优选  

Optimization of Extraction Process for Total Alkaloids from Zanthoxylum armatum by Ultrasonic Assisted Aqueous Two-phase System

作　　者：郭涛[1] 孙莉[1] 黄艳[1] 吕光耀[1] 张丙云[1] 

机构地区：[1]兰州理工大学生命科学与工程学院,兰州730050

出　　处：《中国实验方剂学杂志》2014年第13期8-11,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81360476);甘肃自然科学基金项目(1112RJZA016)

摘　　要：目的:优选超声波辅助双水相体系提取竹叶椒总生物碱的工艺条件。方法:采用超声波辅助丙醇-硫酸铵双水相体系提取竹叶椒总生物碱,选择提取时间、醇水质量比和盐用量为自变量,总生物碱提取率为因变量,通过三因素三水平的响应面试验优选提取工艺。结果:最佳提取工艺为提取时间40 min,醇水质量比0.5∶1,盐用量7.79 g;总生物碱提取率1.704%,与模型预测值(1.711%)偏差很小,纯度19.8%。结论:超声波辅助双水相体系法提取总生物碱具有效率高、纯度高的特点,可避免不稳定或易变性的生物活性分子变性。Objective： To optimize ultrasonic assisted aqueous two-phase system extraction technology of total alkaloids from Zanthoxylum armature. Method： Total alkaloids from Z. armature was extracted by ultrasonic extraction technology coupling with propyl-alcohol ammonium sulfate aqueous two-phase system, taking extracting time, mass ratio of alcohol to water and salt dosage as independent variables, extraction rate of total alkaloids as dependent variable, a three response factors and three levels composite design of response surface methodology was employed to optimize extraction technology. Result： Optimal extraction process was as following： extracting time of 40 rain, mass ratio of alcohol to water 0.5： 1, salt dosage 7.79 g. Under these conditions, extraction rate of total alkaloids was up to 1. 704% , which was agreed with model predicted value （ 1. 711% ） , purity of total alkaloids was 19.8%. Conclusion： Ultrasound assisted aqueous two-phase system for total alkaloids from Z. armatum had advantages of high extraction rate and high purity, which could avoid denaturation of bioactive molecules with instability or volatility.

关 键 词：双水相体系 竹叶椒 总生物碱 响应面试验 超声波提取工艺 回流提取法 

分 类 号：R283.6[医药卫生—中药学] R284.2[医药卫生—中医学]