紫外分光光度法测定温肺化纤汤中各大类成分的含量  

Determination of major components in Wenfeihuaxian formula by ultraviolet spectrophotometry

作　　者：周茂福[1] 胡梦梅[2] 张元兵[1] 兰智慧[1] 刘良徛[1] 

机构地区：[1]江西中医药大学附属医院,江西南昌330006 [2]广州中医药大学第二临床医学院,广东广州510006

出　　处：《时珍国医国药》2014年第6期1283-1285,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81260537);江西省自然科学基金(No.20132BAB205093);江西省卫生厅中医药重点科研计划(No.2012Z004);江西省科技厅支撑计划(No.20111BBG70020-6)

摘　　要：目的利用紫外分光光度法测定温肺化纤汤中总黄酮、总多酚、总皂苷和总多糖的含量。方法采用硝酸铝显色法测定温肺化纤汤中总黄酮的含量;采用Folin-ciocalteu法测定温肺化纤汤中总多酚的含量;采用香草醛-冰醋酸-高氯酸显色法测定温肺化纤汤中总皂苷的含量;采用硫酸苯酚比色法测定温肺化纤汤中总多糖的含量。结果芦丁在25∽200μg·ml-1范围内线性关系良好（r=0.999 0）,测得温肺化纤汤中总黄酮含量为（7.37±0.76）mg·ml-1;没食子酸在10∽100μg·ml-1范围内线性关系良好（r=0.999 5）,测得温肺化纤汤中总多酚含量为（11.83±0.12）mg·ml-1;薯蓣皂苷元在25∽200μg·ml-1范围内线性关系良好（r=0.999 7）,测得温肺化纤汤中总皂苷含量为（20.75±2.43）mg·ml-1;葡萄糖在10∽100μg·ml-1范围内线性关系良好（r=0.999 0）,测得温肺化纤汤中总多糖含量为（154.81±3.00）mg·ml-1。结论紫外分光光度法简捷、准确、灵敏度高,可以很好地测定温肺化纤汤中黄酮类、多酚类、皂苷类和多糖类化合物的含量,其结果为温肺化纤汤的新药研发提供了科学依据。Objective To determine the contents of major components in Wenfeihuaxian formula. Methods The contents of total saponin from Wenfeihuaxian formula were determined by vanillin - ice acetic acid - perchloric method. The contents of total sapo- nin from Wenfeihuaxian formula were determined by vanillin - ice acetic acid - perchloric method. The contents of total saponin from Wenfeihuaxian formula were determined by vanillin - ice acetic acid - perchloric method. The contents of total saponin from Wenfeihuaxian formula were determined by vanillin - ice acetic acid - perchloric method. Results The regression equation of rutin was A =0. 0026C -0. 00152 （r = 0. 999 0）. The results showed that total flavonoids of Wenfeihuaxian formula was （7.37 ± 0.76 ）mg·ml-1 The regression equation of gallic acid was A = 0.0095 C ± 0. 0248 （ r = 0.999 5 ）. The results showed that total polyphenols of Wenfeihuaxian formula was （ 11.83 ± 0.12）mg·ml-1. The regression equation of diosgenin was A = 2. 2540C ± 0.0544 （ r = 0. 999 7）. The results showed that total flavonoids of Wenfeihuaxian formula was （20.75 ± 2.43 ）mg·ml-1. The regression equation of glucose was A = 0.0133C ± 0. 0463 （ r = 0. 999 0）. The results showed that total flavonoids of Wenfeihuax-Jan formula was （154.81 ±3.00） mg·ml-1. Conclusion Ultraviolet spectrophotometry is a simple and quick approach to of Wenfeihuaxian formula with accuracy and sensitivity. The results provide a scientific basis for new drug development of Wenfeihuaxian formula.

关 键 词：紫外分光光度法 温肺化纤汤 总皂苷 总多酚 总黄酮 总多糖 

分 类 号：R284.2[医药卫生—中药学]