超高压诱变小麦突变株系的抗旱综合评价  

Comprehensive Evaluation of Drought Resistance of Wheat Mutant Lines Derived from Ultra-high-pressure Mutagenesis

作　　者：熊建云[1] 张正茂[2] 白婷[2] 卓武燕[1] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100 [2]西北农林科技大学食品科学与工程学院,陕西杨凌712100

出　　处：《西北农业学报》2014年第6期81-87,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家高新技术研究发展计划课题(2011AA100103);陕西省科技统筹创新工程项目(2011KTZB02-01)

摘　　要：通过对超高压诱变小麦突变株系及其野生品种的多项抗旱生理指标进行比较研究,鉴定突变系是否具有抗旱性变异。以偃展4110及其超高压诱变的突变株系为材料,在正常条件和干旱胁迫条件下进行抗旱生理指标测定。结果表明:有些突变系的抗旱系数与未诱变的对照相比呈显著或极显著差异。用主成分分析将超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、脯氨酸(Pro)质量分数、丙二醛(MDA)质量摩尔浓度、光合速率(Pn)、水分利用率(WUE)、相对含水量(RWC)、相对失水速率(RWL)、叶绿素相对含量(SPAD)9个单项的生理指标转换成4个相互独立的综合指标,并通过计算得到这一突变株系的综合抗旱D值。在12个突变系材料中有7个材料的综合抗旱值有显著变化,这些结果说明高压诱变是一项有效的诱变技术,为该技术在抗旱研究中的应用提供依据。To identify the drought resistance variation of wheat mutant lines induced by ultra-highpressure,wheat variety Yanzhan 4110and its mutant lines were used for measuring drought resistance indexes under the conditions of normal watering and drought stress.The results showed that the drought resistance coefficients（DRC）of some of mutations were significant compared with the CK.9single physical indexes,including SOD,POD,Pro,MDA,Pn,WUE,RWC,RWL and SPAD,were converted to 4comprehensive indexes with Principal Component Analysis（PCA）,then calculated the comprehensive drought resistance value（D）.There are 7mutant lines having changed its value D.These results suggest that ultra-high-pressure is an effective induced mutation.It provides the theoretical basis for the drought-resistant research.

关 键 词：超高压诱变 突变株系 主成分分析 抗旱性 

分 类 号：S512.1[农业科学—作物学]