外源水杨酸对盐胁迫下茶树生长及抗氧化酶活性的影响  

Effects of Exogenous Salicylic Acid on Growth and Antioxidant Enzyme Activities of Tea Plant (Camellia sinensis) under Salt Stress

作　　者：周旋[1] 申璐[1] 肖霄[2] 金媛[1] 毛双双[1] 陈良超[1] 肖斌[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100 [2]西北农林科技大学理学院,陕西杨凌712100

出　　处：《西北农业学报》2014年第6期127-133,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家茶叶产业技术体系项目(CARS-23);陕西省科技统筹创新工程计划(2013KTZB02-01-01)

摘　　要：以‘龙井长叶’为材料进行盆栽,对其进行NaCl盐胁迫处理,以未进行盐处理的茶苗为对照,采用叶片喷施水杨酸(SA)的方法,研究不同浓度外源SA(0、0.5、1.0、1.5mmol·L-1)对茶树生长及抗氧化酶活性的影响,探索外源水杨酸影响茶树耐盐性的生理机制。结果表明,盐胁迫下,外源SA能显著提高茶树植株和叶片的鲜质量和干质量;增强超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸-过氧化物酶(APX)等保护酶的活性,抑制过氧化氢酶(CAT)的活性,提高抗坏血酸(AsA)和还原型谷胱甘肽(GSH)的含量,并降低丙二醛(MDA)含量和电解质渗透率,降低O2-和H2O2的含量,且SA最佳处理浓度为1.0mmol·L-1。SA作为一种外源激素,可提高渗透调节能力和抗氧化能力,维持植株水分平衡,清除活性氧,保护膜结构和功能,缓解盐胁迫对茶树的伤害,促进茶树生长,增强茶树的抗盐性。Tea cultivar,‘Longjingchangye＇,was used to study the effects of different concentrations exogenous salicylic acid（0,0.5,1.0,1.5mmol·L^-1）on growth and antioxidant enzyme activities of tea plant（Camellia sinensis）under 40mmol·L^-1 NaCl stress with foliar spray.The aim is to explore effects of salicylic acid on tea salt tolerance.The results indicated that exogenous salicylic acid significantly increased fresh mass and dry mass of tea leaves and all plant,enhanced activities of protective enzymes,such as superoxide dismutase（SOD）,peroxidase（POD）,and ascorbate peroxidase（APX）,but inhibited activity of catalase（CAT）,and increased the content of ascorbic acid（AsA）and glutathione（GSH）,and decreased malondialdehyde（MDA）content and electrolyte leakage,decreased the content of O2-and H2O2.The optimum treatment concentration was 1.0mmol·L^-1.Salicylic acid could be considered as an exogenous hormone to improve osmotic regulation and antioxidation,water balance,scavenging reactive oxygen species（ROS）and membrane stability,alleviating the harmfulness of salt stress,promoting tea plant growth and enhancing salt resistance of tea.

关 键 词：水杨酸 盐胁迫 茶树 生长 抗氧化酶活性 

分 类 号：S571.1[农业科学—茶叶生产加工]