黑米花青素对祼小鼠HER-2阳性乳腺癌肺转移的影响研究  

EFFECTS OF BLACK RICE ANTHOCYANINS ON PULMONARY METASTASIS OF HER-2 POSITIVE BREAST CANCER XENOGRAFT IN NUDE MICE

作　　者：韩彬[1,2] 罗丽萍[2] 陈祥燕[2] 陈玮[2] 彭晓莉[2] 余小平[2] 

机构地区：[1]遵义医学院公共卫生学院,遵义563003 [2]成都医学院公共卫生系,成都610500

出　　处：《营养学报》2014年第3期258-262,共5页Acta Nutrimenta Sinica

基　　金：国家自然科学基金(No.81273074);成都医学院科研基金(No.CYZ12-015);成都医学院学科建设项目(No.CYXK2012010)

摘　　要：目的探讨黑米花青素(black rice anthocyanins,BRACs)对HER-2阳性乳腺癌肺转移的影响。方法32只4 w龄BALB/c雌性裸鼠均分为对照(灭菌水200μl/d,n=16)和BRACs[BRACs 150 mg/kg·d(n=16)]两组,灌胃5 w接种MDA-MB-453乳腺癌细胞,分别在接种3 w和6 w每组各处死8只,取移植瘤和肺组织,解剖显微镜下观察裸鼠肺转移情况,免疫组织化学法检测肺转移瘤增殖,TUNEL法分析肺转移瘤凋亡。结果BRACs组移植瘤体积在17,23,36,39,41d减小(P<0.05);与对照组比,BRACs组6 w肺转移瘤个数显著降低,Ki-67阳性细胞数减少,肺转移瘤TUNEL阳性细胞增多,差异具有统计学意义(P<0.05)。结论BRACs能够抑制MDA-MB-453细胞异种移植瘤向肺转移,该抑制作用可能与BRACs抑制肿瘤细胞的增殖和促进肿瘤细胞凋亡有关。Objective To explore the effects of black rice anthocyanins（BRACs） on pulmonary metastasis of HER-2 positive breast cancer xenograft in nude mice. Methods Thirty-two BALB/c nude mice aged 4 w were randomly divided into control group（200μl sterile purified water, n=16） and BRACs group（BRACs 150 mg/kg·d, n=16）. After intragastric adminstration for 5 w, the body weight and xenografted tumor volume were measured. Eight nude mice were sacrificed when MDA-MB-453 cells were xenografted at 3 and 6 weeks in each group. Thereafter, the xenografted tumors and lung tissues were collected and pulmonary metastasis was detected by dissecting microscope. The expression of Ki-67 in pulmonary metastasis tumor was determined by immunohistochemistry. TUNEL was used to detect the apoptosis cells in pulmonary metastasis tumor. Results The xenograft tumor volume of nude mice fed BRACs was smaller than that of control group at days 17, 23, 36, 39, 41 after xenografted（P〈0.05）. Compared to the control group, the pulmonary node numbers, expression of proliferating cells in pulmonary metastasis tumor decreased significantly. The number of apoptotic cells in pulmonary metastasis tumor increased significantly in BRACs group（P〈0.05）. Conclusion BRACs could inhibit MDA-MB-453 cell pulmonary metastasis. The effect may be associated with the inhibition of pulmonary metastasis tumor cell proliferation and increased tumor cell apoptosis.

关 键 词：黑米花青素 乳腺癌 肺转移 

分 类 号：R737.9[医药卫生—肿瘤]