刺参酸性黏多糖对H22荷肝癌小鼠肿瘤细胞增殖相关基因表达的影响  

EFFECT OF STICHOPUS JAPONICUS ACID MUCOPOLYSACCHARIDE ON CELLULAR PROLIFERATION RELATED GENES EXPRESSION IN MICE BEARING NEOPLASIA OF H22 HEPATOMA CELLS

作　　者：陈丹丹[1] 宋扬[1] 代海华 

机构地区：[1]青岛大学医学院公共卫生学院营养学教研室,青岛266021

出　　处：《营养学报》2014年第3期263-267,272,共6页Acta Nutrimenta Sinica

基　　金：国家自然科学基金资助项目(No.81072295)

摘　　要：目的观察刺参酸性黏多糖(Stichopus japonicus acidic mucopolysaccharide,SJAMP)抑制H22荷肝癌小鼠肿瘤细胞增殖的作用,并探讨其可能机制。方法以H22荷肝癌小鼠为对象,随机分为对照组、5-FU(5-Fluorouracil,氟尿嘧啶20mg/kg)、SJAMP低、中、高剂量(6.25、12.5、25mg/kg)5组。分别采用腹腔注射方式连续给予对应试剂12 d。HE染色观察肿瘤组织病理学改变;分别采用免疫组化法检测肿瘤组织中PCNA、P53、P21、Cyclin D1及CDK4蛋白表达水平;Real-time PCR法检测P53、P21、Cyclin D1及CDK4 mRNA的表达水平。结果 SJAMP各剂量组H22肿瘤组织生长比较缓慢,其中SJAMP高剂量组肿瘤质量与阴性对照组比较差异有统计学意义(P<0.05)。HE染色观察到SJAMP中、高剂量组肿瘤组织较阴性对照组细胞数目少,排列疏松,坏死区多。免疫组化及Real-time PCR结果显示,SJAMP中、高剂量组中与细胞增殖相关基因的PCNA蛋白,P53、Cyclin D1及CDK4蛋白及mRNA的表达较阴性对照组中的表达明显下调(P<0.05),P21蛋白及mRNA的表达明显上调(P<0.05)。结论 SJAMP能够抑制小鼠H22移植瘤的生长。SJAMP发挥抑瘤作用的作用机制可能是其能够调节肿瘤细胞内细胞增殖相关基因与蛋白的表达,以达到抑制肿瘤细胞增殖的目的。[营养学报,2014,36(3):263-267,272]Objective To observe the effect of Stichopus japonicus acid mucopolysaccharide（SJAMP） on cellular proliferation related genes expression in mice bearing neoplasia of H22 hepatoma cells. Methods The mice bearing H22 transplantation tumor were randomly divided into 5 groups ： control group, 5- FU（5-Fluorouracil） control group（20 mg/kg） and SJAMP groups（6.25, 12.5, 25 mg/kg）. Each mouse was received an intraperitoneal injection once daily for 12 days. Tumor histopathological changes were examined by HE. The expressions of PCNA, P53, P21, Cyclin D1 and CDK4 proteins and mRNAs in the H22 xenograft tumor tissues were detected by using immunohistochemical method and real-time PCR. Results The tumor growth in SJAMP groups were slower, with statistical significance in the SJAMP high dose group（P〈0.05）. Compared to the negative control group, tumor cells were less, arranged loosely and more necrotic in SJAMP groups. The PCNA proteins, P53, Cyclin D1 and CDK4 proteins and mRNAs associated with cell proliferation expressions in SJAMP groups were down-regulated obviously（P〈0.05）, and the expression of P21 protein was up-regulated（P〈0.05）. Conclusion SJAMP can inhibit the growth of H22 transplanted tumor. The possible mechanism is that SJAMP can regulate tumor cell proliferation by regulating related genes expression involved in tumor cell proliferation.

关 键 词：刺参酸性黏多糖 H22荷肝癌小鼠 细胞增殖 

分 类 号：R735.7[医药卫生—肿瘤]